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Plasmid#210159PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151A-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::lys1-W151A-GFP::tADH1 (LYS1 Budding Yeast)
UseTagsExpressionYeastMutationW151APromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPD1cR
Plasmid#86970PurposeExpresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coliDepositorInsertPCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, G108C in PCNA1PromoterT7 promoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD2cX
Plasmid#86968PurposeExpresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coliDepositorInsertPCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…PromoterT7 promoterAvailable sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAL007
Plasmid#233866PurposeMoClo-YTK URA multigene plasmid with MCS; NS3 systems; pTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2DepositorInsertpTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRH3175
Plasmid#210177PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1 (ARO7 Budding Yeast)
UseTagsExpressionYeastMutationR33G,G141SPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM4_14L
Plasmid#183063PurposePlasmid expressing a biosensor for acetic acid and pHluorin: BM3R1-Haa1-mTurquoise2 under RET2p, mCherry under HREsYGP1p and sfpHluorin under TDH3p with flanks for genome integration into HO locusDepositorInsertsmCherry
sfpHluorin
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterRET2, Synthetic promoter, and TDH3Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD3cC
Plasmid#86972PurposeExpresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …PromoterT7 promoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pL-Sapphire
Plasmid#176568PurposeExpression of Sapphire for auxotrophic selection in the absence of leucineDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-mWasabi
Plasmid#176551PurposeExpression of mWasabi for auxotrophic selection in the absence of histidineDepositorInsertmWasabi
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-mWasabi
Plasmid#176553PurposeExpression of mWasabi for auxotrophic selection in the absence of uracilDepositorInsertmWasabi
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-mWasabi
Plasmid#176554PurposeExpression of mWasabi for auxotrophic selection in the absence of methionineDepositorInsertmWasabi
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-TagRFP657
Plasmid#176562PurposeExpression of TagRFP657 for auxotrophic selection in the absence of uracilDepositorInsertTagRFP657
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-Sapphire
Plasmid#176566PurposeExpression of Sapphire for auxotrophic selection in the absence of lysineDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-Sapphire
Plasmid#176567PurposeExpression of Sapphire for auxotrophic selection in the absence of uracilDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-Sapphire
Plasmid#176569PurposeExpression of Sapphire for auxotrophic selection in the absence of methionineDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDS2149
Plasmid#184275PurposeTet-on genome insertion systemDepositorInsertCatrTA
UseTagsExpressionYeastMutationPromoterTDH3Available sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCHph
Plasmid#185863PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-YLL058W-lt
Plasmid#176564PurposeExpression of YLL058W for Lanchea thermotolerans for auxotrophic selection in the absence of uracilDepositorInsertYLL058W-lt
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCKan
Plasmid#185862PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-YLL058W-zr
Plasmid#176565PurposeExpression of YLL058W from Zygosaccharomyces rouxii for auxotrophic selection in the absence of uracilDepositorInsertYLL058W-zr
UseTagsExpressionYeastMutationCodon optimised for expression in S.cerevisaePromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only