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Plasmid#218867PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8f
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10
Plasmid#218865PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8s
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2-PcyA-IRES-HO1
Plasmid#131787PurposeTol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70.DepositorInsertPcyA and HO1
UseSynthetic Biology; Zebrafish tol2 transposonTagsMTS (Mitochondrial Targeting Signal from subunit …ExpressionMammalianMutationPromoterhsp70Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHSP90(G97D)-mOFP
Plasmid#228195Purposeexpressed human Hsp90 (G97D mutation) with a mOFP fluorescent protein tagDepositorInsertHSP90AA1(G97D) (HSP90AA1 Human)
UseTagsmOFPExpressionMammalianMutationG97D mutationPromoterCMVAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHD-w+
Plasmid#80927PurposeVector for generating dsDNA donors for HDR. Empty backbone containing a StuI restriction site and GMR-white for identifying backbone incorporation.DepositorInsertGMR-white
UseHigh copy, amp resistanceTagsExpressionMutationPromoterGMR::hsp70Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10
Plasmid#162388PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8f
UseTags6xHisExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10
Plasmid#162383PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8s
UseTags6xHisExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10
Plasmid#162386PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8s
UseTags6xHisExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10
Plasmid#162385PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8f
UseTags6xHisExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10
Plasmid#162387PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
UseTags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS73
Plasmid#110629PurposeCRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicatingDepositorInsertsshort U6 promoter
cas9
tnos terminator
UseCRISPR; Self-replicating in ustilago maydis, conf…TagsN-terminal NLS, C-terminal HA-tag +NLSExpressionBacterialMutationStreptococcus pyogenes gene codon-optimized for U…PromoterU. maydis hsp70 promoter (Kronstad and Leong, 198…Available sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10
Plasmid#162384PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
UseTags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed-w+
Plasmid#80926PurposeVector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.DepositorInsertsLoxP-3xP3-DsRed-LoxP
GMR-white
UseHigh copy, amp resistanceTagsExpressionMutationPromoter3xP3 and GMR::hsp70Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-DsRed-w+
Plasmid#80801PurposeVector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini and GMR-white for identifying backbone incorporation.DepositorInsertspiggyBac-3xP3-DsRed
GMR-white
UseHigh copy, amp resistanceTagsExpressionMutationPromoter3xP3 and GMR::hsp70Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRM14
Plasmid#163693Purposehsp70 promoter inducible expression of DHB:mNeonGreen-P2A-H2B:mScarletDepositorUseUnspecified; Zebrafish expressionTagsmNeonGreen and mScarletExpressionMutationPromoterHSP70Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRM15
Plasmid#163694Purposehsp70 promoter inducible expression of DHB:mScarlet-P2A-H2B:miRFP670DepositorUseZebrafish expressionTagsmScarlet and miRFP670ExpressionMutationPromoterHSP70Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJC-TALE-GFP
Plasmid#46148DepositorTypeEmpty backboneUseTagsGFPExpressionInsectMutationPromoterhsp70Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUAST-FOXO WT
Plasmid#52982Purposeinsect expression of FOXO WTDepositorInsertFOXO (foxo Fly)
UseTagsHAExpressionInsectMutationPromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHACK-QF2
Plasmid#80274PurposeQF2 HACK backbone. Contains QF2-hsp70, but no gRNAsDepositorInsertQF2
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed-attP-w+
Plasmid#80898PurposeVector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.DepositorInsertsattP
LoxP-3xP3-DsRed-LoxP
GMR-white
UseHigh copy, amp resistanceTagsExpressionMutationPromoter3xP3 and GMR::hsp70Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only