We narrowed to 3,830 results for: mak

1. HR_G3BP1-V5-APEX2-GFP

   Plasmid

   #105284

   Purpose

   homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

   Depositor

   Eugene Yeo

   Article

   Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

   Insert

   G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)
   

   Use

   Human homologous recombination donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Icam 2 SaCas9 YAP sgRNA1

   Plasmid

   #99734

   Purpose

   Expresses SaCas9 and a gRNA targeting mouse YAP

   Depositor

   Yu Huang

   Article

   Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

   Insert

   SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Icam 2 SaCas9 YAP sgRNA2

   Plasmid

   #99735

   Purpose

   Expresses SaCas9 and a gRNA targeting mouse YAP

   Depositor

   Yu Huang

   Article

   Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

   Insert

   SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Icam 2 SaCas9 YAP sgRNA3

   Plasmid

   #99736

   Purpose

   Expresses SaCas9 and a gRNA targeting mouse YAP

   Depositor

   Yu Huang

   Article

   Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

   Insert

   SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

   Plasmid

   #169191

   Purpose

   To express nsp10-nsp16 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

   Insert

   14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINB-M1-di-Ub(G76V)-HOIL-1

   Plasmid

   #229539

   Purpose

   Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli

   Depositor

   Bernhard Lechtenberg

   Article

   Wang et al Life Sci Alliance. 2025 Apr 1;8(6):e2025

   Inserts

   Ubiquitin (UBC Human)
   Ubiquitin (UBC Human)
   HOIL-1 (RBCK1 Human)
   

   Use

   Tags

   6xHis-3C

   Expression

   Bacterial

   Mutation

   Changed Gly 76 to Val and changed Gly 76 to Val

   Promoter

   T7

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV2-sgTrp53-sgAtrx-EFS-Cre

   Plasmid

   #189977

   Purpose

   Expresses Cre cDNA and sgRNAs targeting murine Trp53 and Atrx

   Depositor

   William Kaelin

   Article

   Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

   Insert

   sgTrp53, sgAtrx, Cre recombinase
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-MerCreMer-hygro

   Plasmid

   #188982

   Purpose

   Retroviral expression of tamoxifen-inducible Cre recombinase

   Depositor

   William Kaelin

   Article

   Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

   Insert

   Tamoxifen-inducible Cre recombinase
   

   Use

   Cre/Lox and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLBCX-Large-T-antigenK1 mutant

   Plasmid

   #189978

   Purpose

   Retroviral vector used to immortalize human cells

   Depositor

   William Kaelin

   Article

   Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

   Insert

   Large-T-antigenK1 mutant
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC008 - pBR322 with tetR-inducible RFP

    Plasmid

    #79157

    Purpose

    inducible RFP expression vector

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    TetR + RFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC007 - pRFP constitutive expression

    Plasmid

    #79156

    Purpose

    RFP constitutively expressed

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    RFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC002 - LshC2C2 locus into pACYC184

    Plasmid

    #79151

    Purpose

    Endogenous LshC2c2 locus cloned into pACYC184

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    LshC2c2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC003 - LshC2C2 locus into pACYC184 for spacer cloning

    Plasmid

    #79152

    Purpose

    LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    LshC2c2 locus
    

    Use

    Tags

    Expression

    Mutation

    *

    Promoter

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC001 - huLshC2C2-MBP for bacterial expression

    Plasmid

    #79150

    Purpose

    Expresses human codon-optimized LshC2c2 for purification in E. coli

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    C2c2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPbcsx28

    Plasmid

    #89909

    Purpose

    Expresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoter

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    csx28
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBZcsx27

    Plasmid

    #89900

    Purpose

    Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    csx27
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBZCas13b

    Plasmid

    #89898

    Purpose

    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPbcas13b

    Plasmid

    #89906

    Purpose

    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBZCas13b-HEPN

    Plasmid

    #89901

    Purpose

    Bacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R116A/H121A/R1177A/H1182A

    Promoter

    Lac

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZ001 (AacC2c1 locus)

    Plasmid

    #70166

    Purpose

    Expresses AacC2c1 full locus

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    AacC2c1 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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