We narrowed to 11,434 results for: nsf

1. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

   Plasmid

   #70085

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

   Insert

   mutated human serum transferrin (TF Human)
   

   Use

   Tags

   Hexa His tag and N-terminal signal peptide, 4 aa …

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…

   Promoter

   SV40

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. FSF-GFP

   Plasmid

   #163658

   Purpose

   Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR, TMD from ST6GAL1, and luminal domain from TFR in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This chimera contains the cytosolic tail from TFR…

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. F9SS-GFP

   Plasmid

   #163670

   Purpose

   Expresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, and TMD and luminal domain from ST6GAL1 in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This chimera contains the 9 amino acids from the …

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. F9SF-GFP

   Plasmid

   #163671

   Purpose

   Expresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from ST6GAL1 and luminal domain from TFR in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This chimera contains the 9 amino acids from the …

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. F9FS-GFP

   Plasmid

   #163672

   Purpose

   Expresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from TFR and luminal domain from ST6GAL1 in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This chimera contains the 9 amino acids from the …

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPBbsr-6080NES(Epac-sensor)

   Plasmid

   #162487

   Purpose

   hyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   Epac-sensor (RAPGEF3 Human, Synthetic)
   

   Use

   Tags

   YPet and Turquoise2GL

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK649

   Plasmid

   #72399

   Purpose

   Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)

   Depositor

   T. J. Kappock

   Insert

   amidophosphoribosyltransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-89B1

   Plasmid

   #59375

   Purpose

   Inducible expression of 89B1 (AT1G73880) under T7 promoter

   Depositor

   Christopher Anderson

   Insert

   UGT89B1 (UGT89B1 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK639

   Plasmid

   #72398

   Purpose

   Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase, C-terminal His6 tag (EcPurFH6)

   Depositor

   T. J. Kappock

   Insert

   amidophosphoribosyltransferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBacMam8_N_Flag_3C

    Plasmid

    #178818

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    Flag, 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBacMam2_N_His8_eGFP_TEV

    Plasmid

    #178806

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    His8, eGFP, TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBacMam3_N_His8_eGFP_3C

    Plasmid

    #178807

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    His8, eGFP, 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBacMam4_N_Flag_His8_eGFP_TEV

    Plasmid

    #178808

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    Flag, His8, eGFP, TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBacMam7_N_Flag_TEV

    Plasmid

    #178817

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    Flag, TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBacMam5_N_Flag_His8_eGFP_3C

    Plasmid

    #178816

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    Flag, His8, eGFP, 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFastBacMam6_C_3C_His8_Stop

    Plasmid

    #178810

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    3C, His8, Stop codon

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRL528

    Plasmid

    #58495

    Purpose

    helper plasmid for bacterial conjugal DNA transfer

    Depositor

    Peter Wolk

    Article

    Elhai et al Methods Enzymol. 1988;167:747-54.

    Insert

    encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET15bHis6-pG-MNase

    Plasmid

    #124886

    Purpose

    To transform BL21-Gold(DE3) and be induced by IPTG for His-proteinG-MNase fusion protein expression.

    Depositor

    Keji Zhao

    Insert

    protein G-MNase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-21a(+)-LpxA

    Plasmid

    #12684

    Depositor

    SeWon Suh

    Article

    Lee et al Proteins. 2003 Nov 15. 53(3):772-4.

    Insert

    UDP-N-acetylglucosamine acyltransferase (HP1375 Helicobacter pylori)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCB301TOR

    Plasmid

    #164798

    Purpose

    Oomycete expression vector using Agrobacterium-mediated transformation

    Depositor

    Miaoying Tian

    Article

    Wu et al BMC Microbiol. 2016 Sep 6;16(1):204. doi

    Insert

    NPTII
    

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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