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pAW8_13Myc

Plasmid

#110232

Purpose

Cre/Lox C-terminal tagging construct encoding 13 copies of the human c-myc (Myc) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_yEGFP

Plasmid

#110229

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_1XFLAG

Plasmid

#110234

Purpose

Cre/Lox C-terminal tagging construct encoding single copy of the FLAG epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_TAP

Plasmid

#110240

Purpose

Cre/Lox C-terminal tagging construct encoding the Twin Affinity Purification protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_3HA

Plasmid

#110233

Purpose

Cre/Lox C-terminal tagging construct encoding 3 copies of the influenza virus hemagglutunin (HA) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_yECitrine

Plasmid

#110231

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized yellow fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pNX3d-PK3

Plasmid

#78375

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xPK
ura4+ (ura4 Fission Yeast)

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3d-PK6

Plasmid

#78376

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

6xPK
ura4+ (ura4 Fission Yeast)

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3d-PK9

Plasmid

#78377

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

9xPK
ura4+ (ura4 Fission Yeast)

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3a-HA3

Plasmid

#78359

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
kanMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBN27

Plasmid

#35120

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1delta1

Use

Yeast replicating

Mutation

amplicon has a complete deletion of FCY1 ORF, fla…

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pUAST-2x attB

Plasmid

#206362

Purpose

includes two standard UAS cassettes and a third cassette without a promoter

Depositor

Erdem Bangi

Article

Quintero et al Cell Rep. 2023 Nov 28;42(11):113370. doi

Type

Empty backbone

Expression

Insect

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBI/Rabaptin-5

Plasmid

#171851

Purpose

inducible expression of Rabaptin-5 in mammalian cells via co-transfection with pTet-Off.

Depositor

Guangpu Li

Article

Zhu et al PLoS One. 2010 Feb 16;5(2):e9226. doi: 1

Insert

Rabaptin-5 (human)

Expression

Mammalian

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pBUE411-B

Plasmid

#198234

Purpose

pPLTP::BBM cassette in pBUE411

Depositor

Andrea Gallavotti

Article

Chen et al bioRxiv 2022.09.02.506370

Insert

pPLTP::BBM cassette

Use

CRISPR; Plant binary vector

Expression

Plant

Promoter

PLTP

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFGC-pcoCas9

Plasmid

#52256

Purpose

A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

Depositor

Jen Sheen

Insert

35SPPDK:pcoCas9:NOS cassette

Use

CRISPR; Plant expression

Promoter

constitutive 35SPPDK promoter

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pOSIP-KL-mcherry

Plasmid

#116947

Purpose

Integrating mCherry into lambda attB in E. coli chromosome.

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

mcherry

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSR1154

Plasmid

#221653

Purpose

Tetracycline sensor v3 for Staphylococcus aureus

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Inserts

sfGFP cassette
TetR cassette

Use

Synthetic Biology

Expression

Bacterial

Promoter

PXyl-TetO and PZwf

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAd5-B6 ∆E3-GFP

Plasmid

#179201

Purpose

Block 6 with deletion in E3 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified block 6, with deletion in E3 and insertion of GFP expression cassette

Use

Adenoviral

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pUASTP2.1

Plasmid

#13842

Depositor

Ting Wu

Article

Bateman et al Genetics. 2006 Jun . 173(2):769-77.

Insert

mini-white (w Fly)

Expression

Insect

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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HCMV UL97

Plasmid

#26687

Depositor

Robert Kalejta

Article

Kuny et al PLoS Pathog. 2010 . 6(9):.

Insert

UL97 (UL97 Human Cytomegalovirus (HHV-5))

Tags

HA

Expression

Mammalian

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only