We narrowed to 4,135 results for: p2a

1. pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCS

   Plasmid

   #199352

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA-3C

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199364

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187949

   Purpose

   SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   SMASh-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

   Plasmid

   #169336

   Purpose

   Expression of the LT-Fucci(SA) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Fucci(SA) (GMNN, CDT1 Human)
   

   Use

   Tags

   HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMV-Pink Flamindo-p2a-paGFP-bPAC-CAAX

   Plasmid

   #202718

   Purpose

   Mammalian co-expression of soluble Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Pink Flamindo
   paGFP-bPAC-CAAX
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PL752-CLSTN2-LA-p2a-tdTomato-hGHpA-PGK-puroR-CLSTN2-RA

   Plasmid

   #191685

   Purpose

   For knocking p2a-tdTomato into CLSTN2 locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   CLSTN2 homology arms and p2a-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA

   Plasmid

   #191690

   Purpose

   For knocking p2a-EGFP into OTX2 locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   OTX2 homology arms and p2a-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PL752-LMX1A-LA-p2a-tdTomato-hGHpA-PGK-puroR-LMX1A-RA

   Plasmid

   #191683

   Purpose

   For knocking p2a-tdTomato into LMX1A locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   LMX1A homology arms and p2a-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR BFP-guide1

   Plasmid

   #118161

   Purpose

   CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the BFP CDS

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHBS1335 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295S]

    Plasmid

    #107848

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G295S in splicing reporter

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PL452-EN1-LA-p2a-mNeonGreen-hGHpA-PGK-neoR-EN1-RA

    Plasmid

    #191684

    Purpose

    For knocking p2a-mNeonGreen into EN1 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    EN1 homology arms and p2a-mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PL452-CLSTN2-LA-p2a-EGFP-hGHpA-PGK-neoR-CLSTN2-RA

    Plasmid

    #191688

    Purpose

    For knocking p2a-EFGP into CLSTN2 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    CLSTN2 homology arms and p2a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

    Plasmid

    #174529

    Purpose

    Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Inserts

    PylS
    PylT
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHBS1338 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348V]

    Plasmid

    #107852

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G348V in splicing reporter

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHBS1398 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W]

    Plasmid

    #118811

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G368W in CTD of full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHBS1285 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G309F]

    Plasmid

    #107851

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G309F in splicing reporter

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 4xΦ]

    Plasmid

    #107845

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All hydrophobics combined in four clusters in spl…

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHBS1203 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2xΦ]

    Plasmid

    #107844

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All hydrophobics combined in two clusters in spli…

    Promoter

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHBS1339 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348S]

    Plasmid

    #107850

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G348S in splicing reporter

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2+8-CYP2A6(C82A, C439A)-flag-tev-halo-his

    Plasmid

    #227163

    Purpose

    For T-REX experiments of CYP2A6 C82A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).

    Depositor

    Yimon Aye

    Article

    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

    Insert

    CYP2A6 (CYP2A6 Human)
    

    Use

    Tags

    Flag, HaloTag, and His6

    Expression

    Mammalian

    Mutation

    changed cysteine 82 and cysteine 439 to alanine

    Promoter

    CMV and SP6

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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