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pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)

Plasmid

#211863

Purpose

Nuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

3xNLS-HA

Mutation

C492A

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIC8-NLS

Plasmid

#207395

Purpose

Expresses AcrIC8 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC8-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIE2-NLS

Plasmid

#207396

Purpose

Expresses AcrIE2 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIE2-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIC9-NLS

Plasmid

#207397

Purpose

Expresses AcrIC9 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC9-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T3_gRNA

Plasmid

#199389

Purpose

pDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type III sgRNA

Use

Tol2 destination vector

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK128

Plasmid

#200140

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Ubc6-TA (233-250)

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK127

Plasmid

#200139

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Fox2-PTS1 (870-900)

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK123

Plasmid

#200130

Purpose

Type 7 part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

pRS CEN/ARS

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T2_gRNA

Plasmid

#197647

Purpose

pDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type II sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T1_gRNA

Plasmid

#199332

Purpose

pDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type I sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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