We narrowed to 2,532 results for: Kap

1. pDONR223_CGREF1_WT_V5

   Plasmid

   #82879

   Purpose

   Gateway Donor vector containing CGREF1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, Matthew Meyerson

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   CGREF1 (CGREF1 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX_307-SOD1E133K

   Plasmid

   #83447

   Purpose

   Mammalian expression of SOD1E133K

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   E133K

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSOD1E133insTT-AcGFP1

   Plasmid

   #83441

   Purpose

   Expresses SOD1E133insTT in mammalian cells.

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   SOD1 (SOD1 Human)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   E133insTT

   Promoter

   CMV

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSOD1E133Δ-AcGFP1

   Plasmid

   #83419

   Purpose

   Expresses SOD1E133Δ in mammalian cells.

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   SOD1 (SOD1 Human)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   E133 deletion

   Promoter

   CMV

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABEpuro-ERBB2 C299S

   Plasmid

   #40988

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   C299S

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-CMV-PDGFRb-HA

   Plasmid

   #204350

   Purpose

   Expresses wild-type PDGFRb gene. Used for DNA delivery using Adeno-associated Virus.

   Depositor

   Hirofumi Nakatomi

   Article

   Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

   Insert

   Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
   

   Use

   AAV

   Tags

   HA

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

   Plasmid

   #213024

   Purpose

   Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

   Depositor

   Oliver Weichenrieder, Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (238-1061)
   

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Promoter

   T7 / Lac

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

   Plasmid

   #177334

   Purpose

   bacterial expression of active SARS-CoV-2 3C-like proteinase

   Depositor

   Christopher Overall

   Article

   Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
   

   Tags

   Factor Xa site-3xFlag-Myc-6xHis

   Expression

   Bacterial

   Mutation

   silent mutation C to T at position 10546 (elimina…

   Promoter

   T7

   Available Since

   Nov. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR223_HSD17B10_WT_V5

   Plasmid

   #82941

   Purpose

   Gateway Donor vector containing HSD17B10, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, Matthew Meyerson

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   HSD17B10 (HSD17B10 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-PDGFRb559-562del-IRES2-EGFP

    Plasmid

    #204355

    Purpose

    Expresses a mutant PDGFRb gene (559-562 deletion).

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Mutation

    559-562 deletion

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-PDGFRb559-562del-HA

    Plasmid

    #204351

    Purpose

    Expresses a mutant PDGFRb gene (559-562 deletion). Used for DNA delivery using Adeno-associated Virus.

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Use

    AAV

    Tags

    HA

    Mutation

    559-562 deletion

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid

    #224697

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid

    #213026

    Purpose

    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid

    #213030

    Purpose

    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid

    #213031

    Purpose

    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

    Depositor

    Donna Romero, Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    

    Use

    Luciferase

    Tags

    Firefly luciferase antisense intron (3' UTR)

    Expression

    Mammalian

    Promoter

    pTRE bidirectional tet-on

    Available Since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

    Plasmid

    #213025

    Purpose

    Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (1-1275)
    

    Tags

    3C-3xFlag (ORF2p)

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid

    #177335

    Purpose

    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase

    Depositor

    Christopher Overall

    Article

    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Tags

    Factor Xa site-3xFlag-Myc-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    Nov. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid

    #224698

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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