Addgene

We narrowed to 9,116 results for: pho

Showing: 2001 - 2020 of 9116 results

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GST-FOXO3a 1-506aa

Plasmid

#10826

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

GST-FOXO3a 1-506aa (FOXO3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pGEX Vav1-SH2

Plasmid

#46528

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Vav1 (VAV1 Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 661-777

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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GST-FOXO3a TM 1-506aa

Plasmid

#8351

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a 1-506aa (FOXO3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

T32A, S253A, S315A

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pGEX-4T MDM2 S186D

Plasmid

#16239

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Ser186 replaced by Asp

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pDEST51-LRRK2-R1441H

Plasmid

#25083

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Arg 1441 mutated to His

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
p28atDarpp

Plasmid

#108318

Purpose

Expresses tDarpp in E.coli

Depositor

Jamil Momand

Article

Momand et al FEBS Open Bio. 2017 Aug 29;7(9):1328-133

Insert

tDarpp (PPP1R1B Human)

Use

Tags

6 Histadine

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA CrkII Y221F

Plasmid

#50732

Purpose

mammalian expression of CrkII Y221F mutant

Depositor

Kenneth Yamada

Article

Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

Insert

CrkII Y221F (CRK Human)

Use

Tags

Expression

Mammalian

Mutation

Y221F

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pGEX Eat2-SH2

Plasmid

#46423

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Eat2 (SH2D1B Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-132

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-S127/384A

Plasmid

#33076

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S127A, S384A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 D444-500

Plasmid

#19839

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 D444-500 (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 444-500

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-4T MDM2 S166D

Plasmid

#16238

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Ser166 replaced by Asp

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pGEX PLCg1(N)-SH2

Plasmid

#46470

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(N)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 539-664

Promoter

Tac

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5-Myr-FLAG-p110β-DM

Plasmid

#55719

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 beta

Use

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

S205D, K224A

Promoter

T7

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 D500-630

Plasmid

#19841

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 D500-630 (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 500-630

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pNMHCII-C1C2-GFP

Plasmid

#46044

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

Insert

nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12

Plasmid

#39510

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Use

Eukaryotic

Tags

Expression

Mutation

G12V

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pRSET-spa-GCaMP6f

Plasmid

#67563

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Use

Tags

HIS-tag

Expression

Bacterial

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)

Plasmid

#40341

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

Blimp1 promoter (mutated) (PRDM1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Two AP-1 sites at -1813 and -1647 replaced with B…

Promoter

Blimp1

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S127/387A

Plasmid

#33079

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S127A, S387A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3 humanN-Flag SadBS

Plasmid

#66881

Purpose

Mammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadB

Depositor

Maria Alvarado-Kristensson

Article

Eklund et al J Biol Chem. 2014 Aug 1;289(31):21360-73

Insert

SadB (BRSK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only