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T7-5'UTR-bpNLS-BceINT-3'UTR-polyA (CJT483)

Plasmid

#248217

Purpose

bpNLS-BceINT IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-bpNLS-BceINT-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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Dn29 attD pDonor (CJT454)

Plasmid

#248228

Purpose

Dn29 attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

Dn29 attD

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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SacINT attP pDonor (CJT487)

Plasmid

#248227

Purpose

SacINT attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

SacINT attP

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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BceINT attP pDonor (CJT486)

Plasmid

#248226

Purpose

BceINT attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

BceINT attP

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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Bxb1 attP pDonor (CJT453)

Plasmid

#248224

Purpose

Bxb1 attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

Bxb1 attP

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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T7-5'UTR-bpNLS-SacINT-3'UTR-polyA (CJT485)

Plasmid

#248219

Purpose

bpNLS-SacINT IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-bpNLS-SacINT-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-Piv (CJT504)

Plasmid

#248214

Purpose

Piv invertase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

Piv invertase

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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Pa01 attP60 pDonor (CJT460)

Plasmid

#248225

Purpose

Pa01 attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

Pa01 attP

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-ePiv (CJT553)

Plasmid

#248215

Purpose

ePiv invertase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

ePiv invertase

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

ePiv(E257R, E285R, T290R)

Promoter

CMV and T7

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-IS621 (CJT489)

Plasmid

#248213

Purpose

bpNLS-tagged IS621 bridge recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-IS621

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-Bxb1 (EMK569)

Plasmid

#248208

Purpose

Bxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-Bxb1

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pACT-HAB1

Plasmid

#241276

Purpose

Yeast two hybrid vector expressing AD-HAB1

Depositor

Sean Cutler

Article

Park et al Science. 2009 May 22;324(5930):1068-71.

Insert

HAB1 (HAB1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR1 Cys-less

Plasmid

#236274

Purpose

Bacterial expression of bovine arrestin-1 C63A, C128S, C143A, inserted GGT codon after starting ATG

Depositor

Vsevolod Gurevich

Article

Hanson et al Proc Natl Acad Sci U S A. 2006 Mar 28;10

Insert

arrestin-1 (SAG Bovine)

Expression

Bacterial

Mutation

C63A, C128S, C143A, inserted GGT codon after star…

Promoter

lac

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-CCR5-Cys3A

Plasmid

#222313

Purpose

Synchronize the trafficking of CCR5-Cys3A from the ER.

Depositor

Franck Perez

Article

Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

Insert

Streptavidin-KDEL and CCR5-Cys3A fused to SBP-EGFP (CCR5 Human)

Expression

Mammalian

Mutation

C321A, C323A, C324A

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-S528D-S529D-sgEIF3D

Plasmid

#222127

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S528D, S529D

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-CtermDel-sgEIF3D

Plasmid

#222131

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

161 + 527 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-S528N-S529N-sgEIF3D

Plasmid

#222129

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S528N, S529N

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSB278 - pL0_pT16H2 (pro + 5U)

Plasmid

#203889

Purpose

T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T16H2 promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1224 - pL0_pT3R (pro + 5U)

Plasmid

#203892

Purpose

T3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T3R promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1215 - pL0_pDAT (pro + 5U)

Plasmid

#203895

Purpose

DAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

DAT promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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