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pB-DEST (JDW 1205)

Plasmid

#229821

Purpose

A PiggyBac destination vector compatible with gateway cloning system.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Neo-DEST (JDW 940)

Plasmid

#229825

Purpose

A neo selectable, PiggyBac destination vector compatible with gateway cloning system.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-phiC31o (JDW 453)

Plasmid

#229842

Purpose

A CMV-driven Phi31 recombinase.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

phiC31 codon optimized, PhiC31o

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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GvpA-CaM

Plasmid

#223555

Purpose

Full length gas vesicle coat protein A derived from H. salinarum (GvpA) fused to calmodulin.

Depositor

Jean Gariepy

Article

Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

Insert

pET300-GvpACaM

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-Jarid2-polyA

Plasmid

#223811

Purpose

IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

Depositor

Azusa Inoue

Article

Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

Insert

Jarid2 (Jarid2 Mouse)

Use

In vitro transcription

Tags

Flag

Expression

Mammalian

Promoter

CMV, T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pET30-10His-FKBP(f36v)-SNAP

Plasmid

#200282

Purpose

For recombinant expression in E.coli (Rosetta) of a 10His-tag - FKBP(f36v) - SNAPtag fusion protein

Depositor

Kristina Ganzinger

Article

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

FKBP-SNAPtag

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-nucGFP11-3xPax7gRNA

Plasmid

#224570

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

GFP11-H2B

Use

CRISPR

Tags

Histone H2B (nuclear localization)

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHsp70(K71E)-GFP

Plasmid

#228196

Purpose

expressed human Hsp70 (K71E mutation) with a GFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

French et al Proc Natl Acad Sci U S A. 2013 Feb 12;11

Insert

HSP70(K71E) (HSPA1A Human)

Tags

GFP

Expression

Mammalian

Mutation

K71E

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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TadA-8e-eIscBn

Plasmid

#227018

Purpose

Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CMV promoter

Depositor

Dali Li

Article

Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

Insert

bpNLS-TadA-8e-linker-nOgeuIscB-v3-bpNLS

Mutation

D96R/E84R/V159R/H339A

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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IscB-v3

Plasmid

#227015

Purpose

Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CMV promoter

Depositor

Dali Li

Article

Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

Insert

SV40 NLS-OgeuIscB-v3-nucleoplasmin NLS

Mutation

D96R/E84R/V159R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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