Addgene

We narrowed to 2,762 results for: Xpa

Showing: 2041 - 2060 of 2762 results

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pYNL-C1_Rab11a

Plasmid

#65705

Purpose

Expresses YNL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1839

Plasmid

#154325

Purpose

sgRNA Destination Vector for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter

Use

CRISPR

Tags

Expression

Worm

Mutation

F+E mutations in sgRNA

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pICOZ-MAG-M235Q

Plasmid

#218299

Purpose

Mammalian expression vector for expression of MAG transposase mutant

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

M235Q mutant of MAG transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAT-7_PM

Plasmid

#217147

Purpose

Mammalian expression vector for expression of hAT-7_PM transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-7_PM transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-HAT1_AG

Plasmid

#217113

Purpose

Mammalian expression vector for expression of HAT1_AG transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

HAT1_AG transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-hAT-7_PM

Plasmid

#217075

Purpose

Donor plasmid of hAT-7_PM Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of hAT-7_PM transposon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pJW1582

Plasmid

#154312

Purpose

mNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mNeonGreen^SEC^AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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MEIS1A-MIY

Plasmid

#21013

Depositor

Corey Largman

Article

Fischbach et al Blood. 2005 Feb 15. 105(4):1456-66.

Insert

MEIS1A (Meis1 Mouse)

Use

Retroviral

Tags

IRES and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pET-45b-RUNX2(WT)-IDR-mCherry

Plasmid

#145286

Purpose

Bacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(WT)-IDR (RUNX2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1846

Plasmid

#154326

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP^SEC^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

F+E mutations in sgRNA

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1838

Plasmid

#154324

Purpose

sgRNA Destination Vector for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Tags

Expression

Worm

Mutation

F+E mutations in sgRNA

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW2088

Plasmid

#154336

Purpose

Crispr repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP::3xFLAG::10aa linker

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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inhibin-DsRed

Plasmid

#156184

Purpose

Expression plasmid for production of transgenic rainbow trout strain carrying the DsRed gene under control of the inhibin α promoter.

Depositor

Goro Yoshizaki

Article

Iwasaki-Takahashi et al Commun Biol. 2020 Jun 15;3(1):308. doi:

Inserts

rainbow trout inhibin α promoter
rainbow trout β actin 3'UTR

Use

Tags

Expression

Bacterial

Mutation

Promoter

inhibin

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_AnapRS

Plasmid

#174894

Purpose

AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AnapRS

Use

Tags

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ143-ZmUbi

Plasmid

#138107

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(84Q)

Plasmid

#21754

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 84 Glutamines and no…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pCK341

Plasmid

#192640

Purpose

Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dSpRY
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

SoxS has R93A and S101A mutations and dSpRY has s…

Promoter

BBa_J23107 and Sp.pCas9

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pL90-Nat-2xHO

Plasmid

#139069

Purpose

CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

Depositor

Gianni Liti

Article

Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

Insert

Two guide RNAs targeting the HO endonuclease

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

TDH3

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(30Q)

Plasmid

#21753

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 32 amino acids (30 Q…

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only
AttB_ACE2_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190076

Purpose

express human ACE2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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