We narrowed to 2,218 results for: dcas9

1. pXPR_023d sgCiPELO #1

   Plasmid

   #228932

   Purpose

   Expression of dCas9-KRAB and sgRNA targeting PELO

   Depositor

   Francisca Vazquez

   Article

   Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

   Insert

   PELO gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXPR_023d sgCiPELO #8

   Plasmid

   #228934

   Purpose

   Expression of dCas9-KRAB and sgRNA targeting PELO

   Depositor

   Francisca Vazquez

   Article

   Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

   Insert

   PELO gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO5-SgHottip-GFP-CRISPRi

   Plasmid

   #134989

   Purpose

   dCas9-mediated inactivation of HOTTIP in mammalian cells

   Depositor

   Suming Huang

   Article

   Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

   Insert

   HOTTIP (HOTTIP Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD143

   Plasmid

   #119880

   Purpose

   Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pBAD_dCas9sth1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GWF180

   Plasmid

   #133607

   Purpose

   CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9

   Depositor

   Dustin Maly

   Article

   Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

   Insert

   DNCR2-VPR, NS3aH1-tagBFP-SpdCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-Cas9-FCPF

   Plasmid

   #220132

   Purpose

   Expresses Cas9-FCPF in E.coli

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   Cas9-FCPF (cas9 S. pyogenes)
   

   Use

   Tags

   His and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAH18

   Plasmid

   #154397

   Purpose

   Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   NA

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. JPF0535a

   Plasmid

   #124042

   Purpose

   Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. ARB365

   Plasmid

   #124048

   Purpose

   Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. ARB366

    Plasmid

    #124049

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. ARB367

    Plasmid

    #124050

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid

    #73497

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB-P2A-mCherry
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

    Plasmid

    #210709

    Purpose

    This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    U6

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Jun lab tunable CRISPRi strain

    Bacterial Strain

    #86400

    Purpose

    Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

    Depositor

    Suckjoon Jun

    Article

    Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

    Bacterial Resistance

    Spectinomycin and hygromycin

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

16. PX393 Gluconacetobacter diazotrophicus Cas9

    Plasmid

    #68330

    Purpose

    Gluconacetobacter diazotrophicus Cas9

    Depositor

    Feng Zhang

    Insert

    GdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX380 Treponema denticola Cas9

    Plasmid

    #68317

    Purpose

    Treponema denticola Cas9

    Depositor

    Feng Zhang

    Insert

    TdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCas9CR5-NG

    Plasmid

    #120228

    Purpose

    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.

    Depositor

    Christopher Reisch

    Insert

    SpCas9-NG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

    Promoter

    pTet

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9CR4-NG

    Plasmid

    #120225

    Purpose

    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.

    Depositor

    Christopher Reisch

    Insert

    SpCas9-NG
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Mutation

    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

    Promoter

    pTet

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX377 Corynebacter diphtheriae Cas9

    Plasmid

    #68314

    Purpose

    Corynebacter diphtheriae Cas9

    Depositor

    Feng Zhang

    Insert

    CdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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