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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,314 results for: nls

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Showing: 2041 - 2060 of 11314 results

  1. pBT3047

    Plasmid
    #122547
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a N-terminal (sv-40) and C-terminal (egl-13) nuclear localization signal under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_NLS(sv-40)::CemOrange2::NLS(egl-13)
    Tags
    MAPKKKRKVV and MSRRRKANPTKLSENAKKLAKEVEN
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122547-plasmid-map-sequence-id-238211

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  4. pCSnERT(#186)

    Plasmid
    #184057
    Purpose
    to express cyclofen-inducible fusions in mammalian cells or fish eggs
    Depositor
    Sophie Vriz
    Insert
    nls-ERT2
    Use
    Synthetic Biology
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184057-plasmid-map-sequence-id-365952

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  5. pGGD_RT_2NLS

    Plasmid
    #136977
    Purpose
    Goldengate/Greengate module nuclear localization signal
    Depositor
    Klaus Theres
    Insert
    SV40 NLS
    Expression
    Bacterial
    Available Since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136977-plasmid-map-sequence-id-269089

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  7. GANES-DEVD-BNLS

    Plasmid
    #50842
    Purpose
    Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP A and ddGFP B
    Tags
    NES sequence LALKLAGLDIGS placed after ddGFP A an…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50842-plasmid-map-sequence-id-76290

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  12. pDSP33

    Plasmid
    #199375
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199375-plasmid-map-sequence-id-391013

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  13. pRD405

    Plasmid
    #163110
    Purpose
    Expression of mScarlet-I_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mScarlet-I_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163110-plasmid-map-sequence-id-321851

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  14. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid
    #117070
    Purpose
    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection
    Depositor
    Kim Failor
    Inserts
    Cas9
    SacB
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    117070-plasmid-map-sequence-id-231696

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  16. pRD400

    Plasmid
    #163107
    Purpose
    Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_eYFP_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163107-plasmid-map-sequence-id-321848

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  20. pDestTol2CR-QUAS:mNeonGreen-NES-P2A-mKate2-NLS

    Plasmid
    #241912
    Purpose
    Zebrafish transgenesis plasmid encoding cytoplasmic mNeonGreen-NES and mKate2-NLS separated by the P2A peptide, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker
    Depositor
    Balázs Enyedi
    Article
    Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116
    Insert
    QUAS enhancer driving mNeonGreen-NES and mKate2-NLS
    Use
    Zebrafish tol2 transgenesis
    Promoter
    QUAS
    Available Since
    Oct. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    241912-plasmid-map-sequence-id-487154

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Showing: 2041 - 2060 of 11314 results