We narrowed to 2,218 results for: dcas9

1. pCas9CR5

   Plasmid

   #120227

   Purpose

   pCas9CR4 with stop codon to prevent translation of ssrA degradation tag

   Depositor

   Christopher Reisch

   Insert

   SpCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Stop codon inserted before ssrA degradation tag

   Promoter

   pTet

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9CR4-VQR

   Plasmid

   #120224

   Purpose

   pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015

   Depositor

   Christopher Reisch

   Insert

   SpCas9-VQR
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Mutation

   Mutations D1135V/R1335V/T1337R

   Promoter

   pTet

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEG302-SunTagng-22aa

   Plasmid

   #115345

   Purpose

   Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome

   Depositor

   Steven Jacobsen

   Insert

   NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBLO 9.3_bCas9-CP230

   Plasmid

   #121497

   Purpose

   TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p15A

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

   Plasmid

   #115344

   Purpose

   SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

   Depositor

   Steven Jacobsen

   Insert

   gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   March 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBLO 9.2_bCas9-CP199

   Plasmid

   #121496

   Purpose

   TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p15A

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBLO 9.6_bCas9-CP1010

   Plasmid

   #121498

   Purpose

   TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p15A

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBLO 9.15_bCas9-CP1249

   Plasmid

   #121500

   Purpose

   TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p15A

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBLO 9.16_bCas9-CP1282

   Plasmid

   #121501

   Purpose

   TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p15A

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCas9-CR4

    Plasmid

    #62655

    Purpose

    Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

    Depositor

    Kristala Prather

    Article

    Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

    Insert

    Cas9 nuclease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRA03

    Plasmid

    #103141

    Purpose

    Triple gRNA expression separated with 28nt Csy4 motif

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    gRNAs
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHelper_TetR/pTet-Tnpase (camR)

    Plasmid

    #213910

    Purpose

    ATc-inducible himar1C9 transposase expression - cam resistance

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    TetR/ptet ATc-inducible himar1C9 transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHelper_TetR/pTet-Tnpase (carbR)

    Plasmid

    #213911

    Purpose

    ATc-inducible himar1C9 transposase expression - carb/amp resistance

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    TetR/ptet ATc-inducible himar1C9 transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CasRX_Pac

    Plasmid

    #194803

    Purpose

    CasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.

    Depositor

    Alexander Paredez

    Article

    Shih et al Proc Natl Acad Sci U S A. 2022 May 31;11

    Insert

    CasRX
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLY185

    Plasmid

    #184146

    Purpose

    RFP generator controlled by Plux2 promoter

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    rfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Plux2

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLY108

    Plasmid

    #130938

    Purpose

    Engineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plux2

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLY103

    Plasmid

    #130937

    Purpose

    Engineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEA1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plux2

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MTK3b_019

    Plasmid

    #123800

    Purpose

    Encodes dsaCAS9 as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    SadCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MTK3b_018

    Plasmid

    #123799

    Purpose

    Encodes dspCAS9 as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    SpdCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLY76

    Plasmid

    #130963

    Purpose

    Engineered sgRNA-LEA2 (2 BoxB aptamers) generator circuit including promoter Plux2

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEA2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plux2

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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