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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 4,217 results for: Alk

Showing: 2081 - 2100 of 4217 results

  11. pX026

    Plasmid
    #167153
    Purpose
    MoClo-compatible Level 1 (position 4) vector encoding Neonothopanus nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 kb 35S promoter, for expression in plants
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    p35s-nnLuz-ocsT
    Use
    Luciferase
    Tags
    His6-tag
    Expression
    Plant
    Mutation
    Promoter
    p35s
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167153-plasmid-map-sequence-id-335274

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  13. S1 1029 CP ABEMax strategy 2

    Plasmid
    #135358
    Purpose
    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)
    Depositor
    Alex Hewitt
    Article
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Insert
    WT Tada-nSpyCas9 aa 1029-evolved Tada
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135358-plasmid-map-sequence-id-267714

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  16. 289aa ELL2

    Plasmid
    #127266
    Purpose
    For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    Use
    In vitro translation
    Tags
    Expression
    Mutation
    Three Mets (133, 138, 186) to Ileu. Contains 289…
    Promoter
    T7
    Available since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127266-plasmid-map-sequence-id-250216

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  18. HA tagged Sph ELL2 Met133, 138, 186 to Ileu

    Plasmid
    #127271
    Purpose
    For in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th Met
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    ELL2 Met133, 138, 186 to Ileu (ELL2 Human)
    Use
    In vitro translation
    Tags
    Expression
    Mutation
    Met133, 138, 186 to Ileu with HA tag after 8th Met
    Promoter
    T7
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127271-plasmid-map-sequence-id-250013

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  19. HA tagged mid ELL2 M133, 138, 186ILeu

    Plasmid
    #127276
    Purpose
    Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt function
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    ELL2 (ELL2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    M133, 138, 186 to Ileu, HA tag at XbaI after Met …
    Promoter
    pEF1a
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127276-plasmid-map-sequence-id-249998

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  20. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

    Plasmid
    #62580
    Purpose
    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.
    Depositor
    Michael Ruppert
    Article
    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6
    Insert
    SPRED1 (SPRED1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    miR-21 binding site 1 mutant (TAAGCTA --> TAGA…
    Promoter
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2081 - 2100 of 4217 results