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MyrPalm-CKAR

Plasmid

#14862

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Use

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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mcherry-PH

Plasmid

#36075

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

PH domain of PLCdelta1 (PLCD1 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLGB29

Plasmid

#126619

Purpose

Suicide vector for allelic replacement in Bacteroides, inulin selection and aTC-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Tags

Expression

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-C1(2)delta

Plasmid

#21216

Depositor

Tobias Meyer

Article

Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

Insert

GFP-N2-PKCdelta-C1 (Prkcd Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

C1(2) domains of PKC delta (rat) (Lab plasmid WC…

Promoter

Available since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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NK803

Plasmid

#166057

Purpose

Puromycin resistance and LifeAct-mCherry expression for Salpingoeca rosetta

Depositor

Nicole King

Article

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Inserts

Pac puromycin resistance gene
LifeAct-mCherry

Use

S. rosetta expression

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm_P1T_DP-GH023

Plasmid

#90317

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG.PKCepsilon.K/W

Plasmid

#10796

Depositor

Alex Toker

Article

Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

Insert

PKC epsilon (PRKCE Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Kinase dead mutant: K437W (Lys 437 to Trp)

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
B4-4

Plasmid

#111478

Purpose

translational release factor 1

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational release factor 1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTYB2-eIF4B

Plasmid

#37226

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF3

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
C245

Plasmid

#174351

Purpose

Template for making mRNA for overexpressing frog RPL3 (C-HA) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

rpl3.L (rpl3.L Frog)

Use

In vitro transcription template

Tags

HA

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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B1-4

Plasmid

#111466

Purpose

translational elongation factor Tu

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor Tu

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B7-5

Plasmid

#111493

Purpose

translational initiation factor 1

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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B1-5

Plasmid

#111467

Purpose

translational elongation factor Ts

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor Ts

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B1-3

Plasmid

#111465

Purpose

translational release factor 3

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational release factor 3

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
B3-2

Plasmid

#111472

Purpose

translational elongation factor 4

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor 4

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B7-1

Plasmid

#111489

Purpose

translational elongation factor G

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor G

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
B5-5

Plasmid

#111484

Purpose

translational initiation factor 3

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 3

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B3-1

Plasmid

#111471

Purpose

translational initiation factor 2

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
1113 pSG5L HA PTEN D3

Plasmid

#10752

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

D3: S380D, T382D, T383D

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-5aHA

Plasmid

#18032

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5a (Wnt5a Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only