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pCAG-mcherry-WIPI2dK128E-cs-TEV-STREP

Plasmid

#171844

Purpose

Mammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

K128E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLX208-SAE1

Plasmid

#171012

Purpose

Ectopic expression of SAE1

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

untagged SAE1

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLX208-nfBFP

Plasmid

#171013

Purpose

Ectopic expression of nonfluorescent BFP

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

untagged nfBFP

Use

Lentiviral

Expression

Mammalian

Mutation

BFP[H66A]

Promoter

Ef1-alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-Six1

Plasmid

#158532

Purpose

Expresses mouse Six1 in mammalian cells

Depositor

Neil Segil

Article

Menendez et al Elife. 2020 Jun 30;9. pii: 55249. doi: 1

Insert

SIX Homeobox 1 (Six1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe (L) (FA)

Plasmid

#170627

Purpose

For the mammalian expression of mLOV2GIVe lit-mimicking mutation I539E and the GEF-deficient mutation F1685A

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (L) (FA)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSUMO human-cPLA2-C2-domain

Plasmid

#170752

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

Insert

PLA2G4A-C2 domain (PLA2G4A Human)

Tags

SUMO

Expression

Bacterial

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1 Δ5H

Plasmid

#163582

Purpose

express Dcp2 ΔH1 Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

L255A; mutate 440-450, 489-500, 701-708, 827-831,…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2C Δ5H

Plasmid

#163581

Purpose

express Dcp2C Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of aa1-300; mutate aa440-450, aa489-50…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 R14A/K310A

Plasmid

#162655

Purpose

Expresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acids R14 and K310 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-ORF68 C52A

Plasmid

#162660

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C52 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A

Plasmid

#162639

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids K174, R179, and K182 mutated to alani…

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 K435A

Plasmid

#162651

Purpose

Expresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acid K435 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 K450A/R451A

Plasmid

#162653

Purpose

Expresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acids K450 and R451 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFA-DAB1

Plasmid

#162691

Purpose

Expresses DabAB1 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB1 inorganic carbon transport complex

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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HJP-178-IVS-Syn21-CreAB-NrdJ-1N-WPREw

Plasmid

#162473

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreAB

Use

Cre/Lox

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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HJP-208-IVS-Syn21-CreAB-NrdJ-1N-WPREUw

Plasmid

#162478

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreAB

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-195-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREUw

Plasmid

#162476

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreB

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-194-IVS-Syn21-CreA-gp41-1N-WPREUw

Plasmid

#162475

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreA

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-179-IVS-Syn21-NrdJ-1C-CreC-WPREw

Plasmid

#162472

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreC

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-176-IVS-Syn21-CreA-gp41-1N-WPREw

Plasmid

#162470

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreA

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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