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pBTK1052

Plasmid

#209542

Purpose

BTK Type 2 antibiotic resistance cassette for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv 2023.09.19.558440

Insert

Type 2 Kanamycin resistance cassette

Use

Synthetic Biology

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pHACK-Sparse.FRT10-p65.AD

Plasmid

#232832

Purpose

Sparse.FRT10 p65.AD for HACK system

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
p65.AD

Use

CRISPR

Expression

Insect

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pVG329

Plasmid

#227771

Purpose

For genomic replacement of YOR1 gene in budding yeast

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al bioRxiv 2024.02.28.582517

Insert

Genomic sequences flanking YOR1 in budding yeast (YOR1 Budding Yeast)

Expression

Yeast

Mutation

N/A

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-CLIPf

Plasmid

#200787

Purpose

For the mammalian expression of CLIPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

CLIPf-HisTag-FLAG

Tags

CLIPf

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-SNAPf

Plasmid

#200788

Purpose

For the mammalian expression of SNAPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

SNAPf-HisTag-FLAG

Tags

SNAPf

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_CMV_cMyc-VSVG

Plasmid

#212644

Purpose

Lentivirus vector to express cMyc-VSVG

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

VSVG

Use

Lentiviral

Tags

cMyc tag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBEST-PrOR1OR2-MGapt-UTR1-deGFP

Plasmid

#227648

Purpose

PrOR1OR2-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.

Depositor

Richard Murray

Article

Kapasiawala et al bioRxiv 2023.03.22.533877

Insert

MG aptamer (MGapt) and deGFP

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBEST-PTet-MGapt-UTR1-deGFP

Plasmid

#227647

Purpose

pTet-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.

Depositor

Richard Murray

Article

Kapasiawala et al bioRxiv 2023.03.22.533877

Insert

MG aptamer (MGapt) and deGFP

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

Plasmid

#222861

Purpose

This plasmid codes for the OgeuIscB nickase

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

OgeuIscB Nickase

Use

CRISPR

Tags

3X HA and Nucleoplasmin NLS and ITR2

Expression

Mammalian

Mutation

E193A for nickase mutation

Promoter

CMV

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'omega

Plasmid

#223870

Purpose

4G-cloning donor for 3'-Gibson overhang, omega-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3'omega sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-TEV

Plasmid

#223872

Purpose

4G-cloning donor for N-terminal His6-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-3C

Plasmid

#223873

Purpose

4G-cloning donor for N-terminal His6-3C tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-3C

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-FLAG3

Plasmid

#223874

Purpose

4G-cloning donor for N-terminal His6-FLAG3 tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-FLAG3

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_ph

Plasmid

#223857

Purpose

4G-cloning donor for insect promoter and terminator, no tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

polyhedrin promoter and SV40 polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_CMV

Plasmid

#223860

Purpose

4G-cloning donor for mammalian promoter and terminator, no tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

CMV promoter and bGH polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_T7

Plasmid

#223854

Purpose

4G-cloning donor for bacterial promoter and terminator, no tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

T7 promoter and terminator

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-SUMO

Plasmid

#223879

Purpose

4G-cloning donor for N-terminal His6-SUMO tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-SUMO

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_GFP-TEV

Plasmid

#223881

Purpose

4G-cloning donor for N-terminal GFP-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

GFP-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_TS-3C

Plasmid

#223883

Purpose

4G-cloning donor for N-terminal TwinStrep-3C tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

TwinStrep-3C

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_GFP-3C

Plasmid

#223882

Purpose

4G-cloning donor for N-terminal GFP-3C tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

GFP-3C

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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