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pMOD_A5801

Plasmid

#176877

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

35S:Luc+:HSP

Use

Luciferase

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG4E

Plasmid

#176868

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG5

Plasmid

#176869

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_5

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG6E

Plasmid

#176870

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_6E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-1-CmYLCV-6

Plasmid

#176872

Purpose

MoClo Level 0 plasmid, contains CmYLCV-6 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV

Expression

Plant

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2

Plasmid

#176864

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG3

Plasmid

#176866

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_3

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG4

Plasmid

#176867

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

Plasmid

#182854

Purpose

Hetero-tetrmer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mEYFP-mCherry2-mEGFP-mApple

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCm No carrier NikJC199U

Plasmid

#174361

Purpose

Expression plasmid with a TEV removable C-term 8xHis Tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Tags

8xHis and TEV

Expression

Bacterial

Mutation

C199U

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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PROM_Ntpsba_chlo

Plasmid

#163950

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

Insert

Nicotiana tabacum psbA promoter

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3TERM_Ntpsba_rev_chlo

Plasmid

#163932

Purpose

L0 part - terminator

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Nicotiana tabacum psba terminator

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pZA59

Plasmid

#174772

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E-4xMyc, Kmr plant selection marker

Tags

4xMyc

Expression

Plant

Mutation

L17E

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pZA61

Plasmid

#174774

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-6xHA, Kmr plant selection marker

Tags

6xHA

Expression

Plant

Mutation

WT

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pZA64

Plasmid

#174777

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V-6xHA, Kmr plant selection marker

Tags

6xHA

Expression

Plant

Mutation

L17E/D481V

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pZA62

Plasmid

#174775

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V-6xHA, Kmr plant selection marker

Tags

6xHA

Expression

Plant

Mutation

D481V

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pZA60

Plasmid

#174773

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V-4xMyc, Kmr plant selection marker

Tags

4xMyc

Expression

Plant

Mutation

L17E/D481V

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pZA58

Plasmid

#174771

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V-4xMyc, Kmr plant selection marker

Tags

4xMyc

Expression

Plant

Mutation

D481V

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA52

Plasmid

#174765

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V with STOP codon

Use

Unspecified

Mutation

L17E/D481V + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA50

Plasmid

#174763

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V with STOP codon

Use

Unspecified

Mutation

D481V + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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