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pRExT2A-NT-PTy

Plasmid

#232202

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_mScarlet_Convergent

Plasmid

#229790

Purpose

Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13236

Plasmid

#219909

Purpose

The base plasmid of TUNEYALI for TF46

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF46 ( YALI1_E20229g) and homologous arm matching TF46

Expression

Yeast

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13199

Plasmid

#219874

Purpose

The base plasmid of TUNEYALI forTF09

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF09 (YALI1_F29652g) and homologous arm matching TF09

Expression

Yeast

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13218

Plasmid

#219893

Purpose

The base plasmid of TUNEYALI for TF28

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF28 (YALI1_B28150g) and homologous arm matching TF28

Expression

Yeast

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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E547* FL USP7

Plasmid

#229001

Purpose

E547-mutant of human USP7

Depositor

Irina Bezsonova

Insert

USP7 1-546 (USP7 Human)

Tags

6xHis cleavable with thrombin

Expression

Bacterial

Mutation

E547 mutated to stop codon

Promoter

T7

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13239

Plasmid

#219912

Purpose

The base plasmid of TUNEYALI for TF49

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49

Expression

Yeast

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111111

Plasmid

#222456

Purpose

Level 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-miniDFR (Solanum lycopersicum)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW00015

Plasmid

#222446

Purpose

Level 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

VP64-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW91069

Plasmid

#222462

Purpose

Level 1 acceptor, Position 3 containing CBS4-miniDFR::mCherry2-HyP5SM-LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-miniDFR (Solanum lycopersicum)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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