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YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Plasmid

#140155

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCZGY921

Plasmid

#135092

Purpose

Transcriptional GFP reporter of acr-2 (cholinergic) expressing neurons in C. elegans

Depositor

Yishi Jin

Article

Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

Insert

Pacr-2

Tags

GFP and Synthetic Intron

Expression

Worm

Promoter

Pacr-2

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMET17p-CCG 316

Plasmid

#131166

Purpose

For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Tags

CCG

Expression

Yeast

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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kank1a_pJet1.2

Plasmid

#121978

Purpose

for generating kank1a riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1a (si:dkey-146l2.1 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-SsoPCNA3

Plasmid

#107069

Purpose

Expresses S. solfataricus PCNA3 fused to MBP in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Tags

6xHis-thrombin cleavage site and MBP

Expression

Bacterial

Promoter

tac promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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P415 δCLD+Ret2 2α

Plasmid

#73878

Purpose

Swapped helices

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

δCLD+Ret2 2α

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 Ret2LD+δC2α

Plasmid

#73877

Purpose

Swapped helices

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD+δC2α

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

Plasmid

#91945

Purpose

Bacterial expression of maize SAE1 and truncated SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Tags

FLAG and HA

Expression

Bacterial

Mutation

Truncation of ZmSAE2 to mimic a splice variant

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTX203

Plasmid

#89267

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA02

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsDEP1-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pTX202

Plasmid

#89266

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA01

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsDEP1-gRNA01

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pTX200

Plasmid

#89264

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsYSA-gRNA01

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pTX199

Plasmid

#89263

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA02

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsPDS-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-OSF-TRIM5-21R(1-300)

Plasmid

#79029

Purpose

Gateway entry vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5-21R (1-300)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

T7

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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AP1298‐1

Plasmid

#87248

Purpose

gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)

Depositor

Geraldine Seydoux

Article

Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

Insert

gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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AP553‐1

Plasmid

#87240

Purpose

gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)

Depositor

Geraldine Seydoux

Article

Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

Insert

gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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mCm7RNA6A

Plasmid

#84367

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 m7 mutant knockin

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2 m7-gRNA6

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pISH-Meis3

Plasmid

#84312

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Meis3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Meis3 (Meis3 Mouse)

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Alx3

Plasmid

#84305

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Alx3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Alx3 (Alx3 Mouse)

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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TU#1412_tiam-1_cDNA

Plasmid

#82364

Purpose

express tiam-1 cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

tiam-1 cDNA (tiam-1 Nematode)

Expression

Worm

Promoter

none

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Dmbx1

Plasmid

#84308

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dmbx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Dmbx1 (Dmbx1 Mouse)

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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