We narrowed to 7,193 results for: Ank

1. pX458-Ef1a-Cas9-H2B-GFP

   Plasmid

   #159654

   Purpose

   Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-C1 SNX9 RYK mutant R286A Y287A K288A

   Plasmid

   #161993

   Purpose

   Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   SNX9 (Snx9 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R286A Y287A K288A

   Promoter

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINDUCER11 (miR-RUG)

   Plasmid

   #44363

   Purpose

   Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

   Depositor

   Thomas Westbrook

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Insert

   PheS Gly294
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-BiFC

   Plasmid

   #87856

   Purpose

   pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Inserts

   Encodes for MCS & C-term half of mVenus
   Encodes for MCS & N-term half of mVenus
   

   Use

   Tags

   BiFC C-terminal fragment

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_6xHis-PARP1-Zn2

   Plasmid

   #174805

   Purpose

   Bacterial expression of isolated PARP1 Zn2 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Zn2 (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_6xHis-PARP1-Zn3

   Plasmid

   #174806

   Purpose

   Bacterial expression of isolated PARP1 Zn3 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Zn3 (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBa.MARK2-Halo

   Plasmid

   #224414

   Purpose

   Mammalian expression of MARK2

   Depositor

   Marvin Bentley

   Article

   Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

   Insert

   MARK2 (MARK2 Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458-Ef1a-Cas9-H2B-mCherry

   Plasmid

   #159655

   Purpose

   Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBR322-TIMER

   Plasmid

   #103056

   Purpose

   medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

   Depositor

   Dirk Bumann

   Article

   Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

   Insert

   TIMER-bac
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutations: S4T and S179T

   Promoter

   constitutive ybaJp promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-MED17

    Plasmid

    #15410

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED17 (MED17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4.10/RSV_SF3B3-miniXon_Luciferase

    Plasmid

    #174660

    Purpose

    Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.

    Depositor

    Beverly L. Davidson

    Article

    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

    Insert

    miniXon cassette
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Kozak ATG initiation sequence in middle exon

    Promoter

    RSV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. eDrIR1

    Plasmid

    #183998

    Purpose

    Optically controlled eDrIR1 expression in mammalian cells

    Depositor

    Dmitry Gerashchenko, Vladislav Verkhusha

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrIR1 (INSR Human)
    

    Use

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1-CAT

    Plasmid

    #173943

    Purpose

    Bacterial expression of isolated PARP1 CAT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CAT (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-2E2-HA-mCh

    Plasmid

    #129596

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIL6-Nluc-hbb

    Plasmid

    #239843

    Purpose

    Template for in vitro transcription of secreted nanoluciferase, with interleukin 6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.

    Depositor

    Catherine Jopling

    Article

    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

    Insert

    Secreted nanoluciferase, with interleukin 6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    T7 (included as part of insert)

    Available since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV9-null

    Plasmid

    #200182

    Purpose

    Expresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 site

    Depositor

    Aravind Asokan

    Article

    Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

    Insert

    AAV9 Cap w/ nonsense mutation at VR8
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Replaced amino acids 586-592 in Cap with nonsense…

    Promoter

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. ER-NFAST

    Plasmid

    #233596

    Purpose

    Expression of NFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    NFAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXL001-TQM IL13 CAR

    Plasmid

    #154054

    Purpose

    Lentiviral expression of TQM IL13 CAR for CAR-T therapy

    Depositor

    Xiaojun Lian

    Article

    Kim et al Bioact Mater. 2020 May 7;5(3):624-635. d

    Insert

    TQM IL13 CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28_6xHis-PARP1delBRCT

    Plasmid

    #173938

    Purpose

    Bacterial expression of PARP1 lacking BRCT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delBRCT (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 374-520; V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hysn-flex-dsRed-shscramble

    Plasmid

    #71383

    Purpose

    Expresses scramble shRNA Cre-dependently by flex switch

    Depositor

    William Wisden

    Article

    Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

    Insert

    human synapsin promoter-flex switch-dsRed-shscramble
    

    Use

    AAV, Cre/Lox, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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