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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 76,217 results for: cloning

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Showing: 2101 - 2120 of 76217 results

  1. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100903-plasmid-map-sequence-id-196607

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  2. EBFP-C1

    Plasmid
    #54738
    Purpose
    Localization: C1 Cloning Vector, Excitation: 387, Emission: 450
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    EBFP
    Expression
    Mammalian
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    54738-plasmid-map-sequence-id-256475

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  4. pVITRO1-Trastuzumab-IgG2/κ

    Plasmid
    #61884
    Purpose
    Expresses HER2/neu receptor specific humanized IgG2/κ antibody isotype (Trastuzumab-IgG2/κ)
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Gamma 2 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61884-plasmid-map-sequence-id-207869

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  5. pVITRO1-dV-IgG1/λ

    Plasmid
    #52214
    Purpose
    Human immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin lambda constant region
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52214-plasmid-map-sequence-id-432867

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  6. pET mClover LIC cloning vector (H6-mClover)

    Plasmid
    #86931
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-mClover
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and mC…
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86931-plasmid-map-sequence-id-174751

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  7. Clover2-N1

    Plasmid
    #54537
    Purpose
    Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Clover2
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    54537-plasmid-map-sequence-id-127234

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  8. Dronpa3-N1

    Plasmid
    #54682
    Purpose
    Localization: N1 Cloning Vector, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Dronpa3
    Expression
    Mammalian
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    54682-plasmid-map-sequence-id-139033

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  11. mKeima-Red-C1

    Plasmid
    #54546
    Purpose
    Localization: C1 Cloning Vector, Excitation: 440, Emission: 620
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mKeima-Red
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54546-plasmid-map-sequence-id-256470

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  12. pRAN473

    Plasmid
    #120813
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Publication
    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1
    Insert
    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    Expression
    Bacterial
    Promoter
    Ptet
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120813-plasmid-map-sequence-id-235308

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  14. pLK89 - Edit-directing plasmid cloning insert

    Plasmid
    #98846
    Purpose
    Contains the insert used for step 2 of edit-directed plasmid cloning
    Depositor
    Leonid Kruglyak
    Insert
    KanR
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Changed sequence to remove a MluI cut site
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    98846-plasmid-map-sequence-id-189230

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  15. AcGFP1-N1

    Plasmid
    #54705
    Purpose
    Localization: N1 Cloning Vector, Excitation: 475, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    AcGFP1
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54705-plasmid-map-sequence-id-162699

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Showing: 2101 - 2120 of 76217 results