We narrowed to 10,582 results for: plasmids 101

1. mApple-Keratin-17

   Plasmid

   #54915

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Intermediate Filaments, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

2. mPlum-Keratin-17

   Plasmid

   #55980

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mPlum

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

3. USP08 (2A9U)

   Plasmid

   #25224

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   usp08.hs.0001-0142 (USP8 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Dimerization Domain, contains amino acids 8-138

   Promoter

   Available Since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pCSRNP3.1.0-gDNA

   Plasmid

   #132472

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   CSRNP3 (CSRNP3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mKOet-Keratin-17

   Plasmid

   #57927

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 548, Emission: 561

   Depositor

   Michael Davidson

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mKOet

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

6. mKO-Keratin-17

   Plasmid

   #57845

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 548, Emission: 561

   Depositor

   Michael Davidson

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mKO

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. mTurquoise-Keratin-17

   Plasmid

   #55564

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 434, Emission: 474

   Depositor

   Michael Davidson

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mTurquoise

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pZ P (cHS4)X4 OCT4p eGFP SV40pA v2

   Plasmid

   #115518

   Purpose

   Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.

   Depositor

   Catherine Verfaillie

   Article

   Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

   Insert

   eGFP
   

   Use

   AAV; Donor plasmid for recombinase-mediated casse…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2

   Plasmid

   #112668

   Purpose

   Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.

   Depositor

   Catherine Verfaillie

   Article

   Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

   Insert

   tdT
   

   Use

   AAV; Donor plasmid for recombinase-mediated casse…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZ:F3-P CAGGS tdTPH-F

    Plasmid

    #112667

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows constitutive expression of your protein of interest.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FDPS

    Plasmid

    #39131

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FDPS (FDPS Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. CNDP1

    Plasmid

    #25128

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CNDP1_01 (CNDP1 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. Human CRISPR Metabolic Gene Knockout Library

    Pooled Library

    #110066

    Purpose

    Encodes gRNAs for targeting metabolic genes in CRISPR screens.

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Use

    Lentiviral

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

14. plRL118

    Plasmid

    #163632

    Purpose

    Renilla luciferase reporter plasmid; Giles integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mycobacterial Optimized Promoter (MOP)

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT3-EF1aH

    Plasmid

    #180149

    Purpose

    This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSBL_DK187

    Plasmid

    #156423

    Purpose

    Insect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein
    

    Use

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-SpCas9(SKNCKS)-P2A-EGFP (RAS2951)

    Plasmid

    #223101

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SKNCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(SKNCKS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(SKNCKS)=D1135S, S1136K, G1218N, E1219C, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid

    #112669

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Kv1.1 K+ channel scFv [K20/78]

    Plasmid

    #190486

    Purpose

    Mammalian Expression of Kv1.1 K+ channel scFV. Derived from hybridoma K20/78.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Kv1.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcna1 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCHC042 (∆phaCAB)

    Plasmid

    #226208

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for phaCAB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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