We narrowed to 30,775 results for: Ide

1. pGEX-THTAP-VPS9D1, residues 1-97

   Plasmid

   #180640

   Purpose

   Bacterial expression for VPS9D1 MIT domain, residues 1-97. Has N-terminal HIS-GST tag. Internal ID: WISP20-89.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   VPS9D1 (C16orf7 Human)
   

   Tags

   HIS-GST

   Expression

   Bacterial

   Mutation

   Residues 1-97

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA528 CHMP4C, residues 156-233, no Cys

   Plasmid

   #180642

   Purpose

   Bacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP4C (CHMP4C Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   residues 316-366

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA528 IST1, residues 315-360, no Cys

   Plasmid

   #180643

   Purpose

   Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   residues 315-360

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCA528 KATNA1 MIT residues 1-79

   Plasmid

   #180614

   Purpose

   Bacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-79

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCA528 KATNA1 MIT residues 1-79, V55D

   Plasmid

   #180615

   Purpose

   Bacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-79, V55D mutation

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCA528 KATNAL1 MIT residues 1-87

   Plasmid

   #180618

   Purpose

   Bacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNAL1 (KATNAL1 Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-87

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-THTAP NRBF2 MIT residues 1-111

   Plasmid

   #180621

   Purpose

   Bacterial expression for NRBF2 MIT domain, residues 1-111. Has N-terminal HIS-GST. Internal ID: WISP20-88.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   NRBF2 (NRBF2 Human)
   

   Tags

   HIS-GST

   Expression

   Bacterial

   Mutation

   Residues 1-111

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCA528 CHMP1B 4-199

   Plasmid

   #115325

   Purpose

   Expresses residues 4-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-23

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. S2 1058 AIDx no UGI

   Plasmid

   #135349

   Purpose

   Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx-ID-1058
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. S2 1058 APOBEC3 no UGI

    Plasmid

    #135350

    Purpose

    Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-rAPOBEC3-ID-1058
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dscam1_7.27.26 EC10-Fc-His

    Plasmid

    #72189

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.26
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Dscam1_7.27.23 EC10-Fc-His

    Plasmid

    #72187

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.23
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Dscam1_7.27.26 EC10-AP-His

    Plasmid

    #72063

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.26
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Neo1.e-Fc-His

    Plasmid

    #72093

    Purpose

    Expresses the extracellular region of the Neogenin 1, isoform e protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Neo1.e (Neo1 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pet151D-TOPO-BROX 1-411; K141E

    Plasmid

    #89865

    Purpose

    Expresses full length human BROX with mutation K141E in bacteria; internal ID WISP11-299

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    mutated lysine 141 to glutamic acid

    Promoter

    T7

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pet151D-TOPO-BROX 1-411; L212D

    Plasmid

    #89864

    Purpose

    Expresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    mutated leucine 212 to aspartic acid

    Promoter

    T7

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GST-CHMP4A (205-222) L217A (pGEX2T(TEV))

    Plasmid

    #80638

    Purpose

    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222; Changed Leucine 217 to Alanine

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GST-CHMP4A (205-222) W220A (pGEX2T(TEV))

    Plasmid

    #80637

    Purpose

    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222; Changed Trp 220 to Ala

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GST-CHMP4A (205-222) L214A (pGEX2T(TEV))

    Plasmid

    #80639

    Purpose

    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222; Changed Leucine 214 to Alanine

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Plxnb1(S)-Fc-His

    Plasmid

    #72127

    Purpose

    Expresses the N-terminal extracellular region of the PlexinB1 protein following proteolytic cleavage (ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxnb1 (Plxnb1 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge