We narrowed to 3,335 results for: UBC

1. pCIG1_UL136_25kDa_Myc

   Plasmid

   #74954

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)

   Expression

   Mammalian

   Mutation

   delta 1-77, M100A M128A

   Promoter

   CMV, Lentiviral LTR

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUP95Q15ADSKGW (D7)

   Plasmid

   #74030

   Purpose

   lentiviral expression of Psd95-Q15A deltaSK-EGFP fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95 (DLG4 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Q15A, delta SH3-GK (deletion of the fragment betw…

   Promoter

   hUbC

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCR4-*mRFP

   Plasmid

   #47118

   Purpose

   Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.

   Depositor

   Stephen Ekker

   Article

   Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

   Insert

   *mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No start codon

   Promoter

   Lac

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. p198 Dync1i2.F (Ex1a)

   Plasmid

   #26448

   Depositor

   Elizabeth Fisher

   Article

   Kuta et al PLoS One. 2010 . 5(7):e11682.

   Insert

   cytoplasmic dynein 1 intermediate chain 2 isoform F (exon 1a) (Dync1i2 Mouse)
   

   Use

   Subcloning and sequencing

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   March 24, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pUltra-MDH1-ME1

   Plasmid

   #184465

   Purpose

   Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Inserts

   Malate dehydrogenase 1 (MDH1 Human)
   Malic Enzyme 1 (ME1 Human)
   

   Use

   Lentiviral

   Tags

   fused to T2A

   Expression

   Mammalian

   Mutation

   deletion of stop codon

   Promoter

   UbC

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ubiquiton In Vitro Kit

   Plasmid Kit

   #1000000242

   Purpose

   The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.

   Depositor

   Helle Ulrich

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

7. PGK-DTAG-NTERM-GFP

   Plasmid

   #185775

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Expression

   Mutation

   none

   Promoter

   PGK

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PGK-DTAG-CTERM-GFP

   Plasmid

   #185770

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Expression

   Mutation

   none

   Promoter

   PGK

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX304-IRES-Td

   Plasmid

   #176296

   Purpose

   pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites

   Depositor

   William Kaelin

   Article

   Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti NL

    Plasmid

    #113450

    Purpose

    Lentiviral NanoLuc control expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    NanoLuc
    

    Use

    Lentiviral and Luciferase

    Tags

    cmyc

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PGK-SMASH-CTERM-GFP

    Plasmid

    #185776

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, SMASh

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV3flag8HOIL-1L

    Plasmid

    #50016

    Purpose

    Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.

    Depositor

    Martin Dorf

    Article

    Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

    Insert

    RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
    

    Use

    Tags

    3 x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. PGK-IKZF3-CTERM-GFP

    Plasmid

    #185779

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, IKZF3 minidegron

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PGK-AID-CTERM-GFP

    Plasmid

    #185778

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, AID

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PGK-HALO-CTERM-GFP

    Plasmid

    #185777

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, Halo

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PGK-SMASH-NTERM-GFP

    Plasmid

    #185771

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, SMASh

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PGK-HALO-NTERM-GFP

    Plasmid

    #185772

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, Halo

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PGK-IKZF3-NTERM-GFP

    Plasmid

    #185774

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, IKZF3 minidegron

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLX304-IRES-GFP

    Plasmid

    #176288

    Purpose

    pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PGK-AID-NTERM-GFP

    Plasmid

    #185773

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, AID

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge