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pAAV-CAG-RiboL1-jGCaMP8m

Plasmid

#192613

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pACAGW-ChR2-Venus-AAV

Plasmid

#20071

Purpose

AAV-mediated expression of ChR2-Venus for neuronal excitation

Depositor

Karel Svoboda

Article

Leopoldo et al Nature. 2009 Jan 18.

Has Service

AAV1 and AAV9

Insert

Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)

Use

AAV

Tags

Venus

Expression

Mammalian

Promoter

CAGG

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-AAVS1_sgRNA

Plasmid

#183890

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6s

Plasmid

#111262

Purpose

For enriched expression of GCaMP6s in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV1

Insert

axon-GCaMP6s

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-DIO-APEX2NES

Plasmid

#79907

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

APEX2NES

Use

AAV and Cre/Lox

Tags

V5

Expression

Mammalian

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-PiGM-Iq(eGFP)

Plasmid

#221602

Purpose

A recombinant AAV2 plasmid encoding the PiGM-Iq system with CRY2PHR, CIBN and EGFP as expression marker. hSynapsin promoter for panneuronal expression.

Depositor

John Lin

Insert

PiGM-Iq (eGFP)

Use

AAV

Mutation

RGS2 1-53 truncation

Promoter

Human Synapsin

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Coff/FonEYFP

Plasmid

#55652

Purpose

Cre-off/Flp-on EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV; Cre off/flp on eyfp

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pACAGW-H2B-PAGFP-AAV

Plasmid

#33000

Depositor

Massimo Scanziani

Article

Lien et al Front Neural Circuits. 2011;5:16. Epub 2

Insert

H2B-PAGFP (H2BC21 Human, Aequorea victoria)

Use

AAV; Adeno associated virus

Tags

PAGFP

Expression

Mammalian

Available Since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jGCaMP8m

Plasmid

#172719

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV ORANGE Dlg4-HaloTag KI

Plasmid

#139656

Purpose

Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and HaloTag donor

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-H2BmTagBFP2-oG

Plasmid

#217976

Purpose

AAV transfer plasmid to express H2B-BFP and codon-optimized Rabies G protein

Depositor

Edward Callaway

Article

Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

Inserts

H2BmTagBFP2
oG

Use

AAV

Tags

mTAGBFP2

Promoter

hSyn

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-mRuby2

Plasmid

#104058

Purpose

AAV expression of Cre-dependent reporter mRuby2

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

mRuby2

Use

AAV

Promoter

CAG

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-hfCas13d-HA-mCherry-pA

Plasmid

#233029

Purpose

To Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d-mCherry

Use

AAV

Tags

mCherry

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hSncg-EGFP

Plasmid

#153198

Purpose

Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-WHaloCaMP1a-EGFP

Plasmid

#205309

Purpose

Production of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

AAV

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-SYP1-miniSOG-citrine

Plasmid

#50971

Purpose

AAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

Synaptophysin-miniSOG-citrine (Syp Rat)

Use

AAV

Tags

citrine and miniSOG

Promoter

human synapsin promoter

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mCherryP2ACre

Plasmid

#161773

Purpose

Flp-Dependent mCherry and Cre expression

Depositor

Baoji Xu

Article

An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

Insert

mCherry-P2A-Cre

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.S29W.A95K.mIRFP670nano3

Plasmid

#244915

Purpose

iATPSnFR2.S29W.A95K variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.A95K.mIRFP670nano3

Use

AAV

Mutation

S29W.A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-jGCaMP7f-WPRE

Plasmid

#203848

Purpose

Expression of GCaMP7f in CaMKII expressing cells

Depositor

Ivo Spiegel

Article

Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

Insert

jGCaMP7f

Use

AAV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Ace-mNeon2

Plasmid

#195518

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

Synapsin

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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