We narrowed to 12,168 results for: nls

1. GFP-NPM NESM-NLS1D

   Plasmid

   #13288

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   NPM (NPM1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   L100A, L102A, deletion of aa134-aa142

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pGEX-4T1 NLS-GFP

   Plasmid

   #129441

   Purpose

   bacterial expression of GFP

   Depositor

   Oskar Laur

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mTubulin NLS-mCherry-LEXY

   Plasmid

   #182597

   Purpose

   Drosophila integration plasmid expressing NLS-mCherry-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   mCherry
   

   Tags

   LEXY and NLS

   Expression

   Insect

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGTag-NLS-TagRFP-B-actin

   Plasmid

   #117806

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-opCas12a-Ultra-6xNLS

   Plasmid

   #209024

   Purpose

   Lentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   opCas12a-Ultra-6xNLS-Flag-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and MYC

   Expression

   Mammalian

   Mutation

   E174R/M537R/S542R/F870L

   Promoter

   EF-1a

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUW-TetO-DsRed-cNLS

   Plasmid

   #138533

   Purpose

   Lentiviral inducible expression of DsRed-Express2 with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   DsRed-Express2
   

   Use

   Lentiviral

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Promoter

   TRE-CMV

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-TetR-NLS-VP16

   Plasmid

   #103834

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   EGFP-TetR-NLS-VP16 (tetR )
   

   Expression

   Mammalian

   Promoter

   SV2

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 416Gal-SV40NLS-FUS-YFP

   Plasmid

   #29595

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   NLS-FUS (FUS Human)
   

   Tags

   SV40 NLS and YFP

   Expression

   Yeast

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pGTag-NLS-eGFP-B-actin

   Plasmid

   #117812

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80067

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. NLS-FUS-Citrine-PixE

    Plasmid

    #111506

    Purpose

    For the nuclear localization of the PixELL system

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    PixE (slr1693 )
    

    Use

    Lentiviral

    Tags

    Citrine, FUS, and NLS

    Promoter

    SFFV

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PET-B-HypaSpCas9-NLS-6xHis

    Plasmid

    #207386

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-mCherry-TSC2(AA,3Q)-NLS

    Plasmid

    #192445

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-mCherry-TSC2-NLS

    Plasmid

    #192441

    Purpose

    Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2-NLS (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-mCherry-TSC2(AA)-NLS

    Plasmid

    #192443

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. A3B-Cas9n-UGI-NLS

    Plasmid

    #198889

    Purpose

    Express full-length A3B BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-NLS-gs10-cGR2176

    Plasmid

    #188258

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cGR2176
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. nGR2175-gs10-NLS-VPR

    Plasmid

    #188264

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nGR2175
    

    Expression

    Mammalian

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-NLS-FRB-VP16

    Plasmid

    #210515

    Purpose

    expresses NLS-FRB-VP16 component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NLS-FRB-VP16
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE-puro-NLS-LSSmKate1

    Plasmid

    #31873

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS-LSS-mKate1
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Available Since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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