Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20889||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeMammalian Expression, Mouse Targeting
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name7XTCF binding sites, Siamois minimal promoter, NLS-lacz
SpeciesX. laevis (frog); E. coli
Insert Size (bp)3700
Mutation7 multimerized TCF-binding elements cloned upstream of the xSiamois minimal promoter, driving expression of NLS-lacZ (nuclear localized beta-galactosidase)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Wnt/beta-catenin responsive promoter in mouse tissues (used to derive the BAT-gal mouse strain), Xenopus embryos and human cell lines
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BAT-gal was a gift from Stefano Piccolo (Addgene plasmid # 20889 ; http://n2t.net/addgene:20889 ; RRID:Addgene_20889)
For your References section:Mapping Wnt/beta-catenin signaling during mouse development and in colorectal tumors. Maretto S, Cordenonsi M, Dupont S, Braghetta P, Broccoli V, Hassan AB, Volpin D, Bressan GM, Piccolo S. Proc Natl Acad Sci U S A. 2003 Mar 18. 100(6):3299-304. 10.1073/pnas.0434590100 PubMed 12626757
Map uploaded by the depositor.