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457 p3E-2A-EGFPpA

Plasmid

#195967

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-EGFP

Use

Other

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEBFP2-Nuc

Plasmid

#14893

Purpose

Mammalian expression of EBFP2 blue fluorescent protein with NLS

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

pEBFP2-Nuc

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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mCherry-VP40

Plasmid

#74421

Purpose

Mammalian expression of Ebolavirus matrix protein VP40 with an mCherry fusion

Depositor

Judith White

Article

Shoemaker et al PLoS One. 2013;8(2):e56265. doi: 10.1371

Insert

VP40

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCK017 pDEST exorh:EGFP

Plasmid

#195983

Purpose

Transgenesis backbone

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::rtTA3-bGHpA

Plasmid

#62445

Purpose

MXS_chaining vector with CMV::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with CMV promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pPEG

Plasmid

#162466

Purpose

Entry vector for gRNA insertion in plant prime editing expression vector

Depositor

Adam Bogdanove

Article

Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

Type

Empty backbone

Use

Entry vector for grna

Promoter

CmYLCV

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

Plasmid

#126552

Purpose

Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

Depositor

Jonathan Karn

Article

Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

Insert

HIV-1 vector pNL4-3

Use

Lentiviral

Tags

CD8a and GFP

Mutation

Delta gag/pol

Promoter

HIV LTR

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52

Plasmid

#98678

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Dimer cleaved

Plasmid

#198199

Purpose

FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mVenus-T2A-mTurquoise2

Expression

Mammalian

Mutation

A206K both

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST exorh:mCherry

Plasmid

#195989

Purpose

Transgenesis backbone

Depositor

Christian Mosimann, Nicolas Rohner

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-rictor

Plasmid

#73387

Purpose

Expresses HA and YFP tagged Rictor

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)

Tags

HA and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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IO001: pMVP (R4-R3) eGFP

Plasmid

#121730

Purpose

pMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IG703: pMVP (L1-R5) TRE promoter

Plasmid

#121697

Purpose

pMVP L1-R5 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IY704: pMVP (L1-R5) EF1a promoter

Plasmid

#121693

Purpose

pMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pET11a-4SS-HEWL

Plasmid

#39233

Depositor

Harald Schwalbe

Article

Schlorb et al J Biomol NMR. 2005 Oct;33(2):95-104.

Insert

Hen egg white lysozyme (LYZ Chicken)

Expression

Bacterial

Mutation

N-terminal Met

Promoter

T7

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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neurod1:CaMPARI

Plasmid

#173479

Purpose

CaMPARI is driven by -5kb neurod1 promoter.

Depositor

John Rawls

Article

Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

Insert

CaMPARI

Use

Zebrafish expression

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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human MTAP

Plasmid

#64077

Purpose

Expression of human 5'-methylthioadenosine phosphorylase

Depositor

Vern Schramm

Article

Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

Insert

5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)

Tags

Histidine tag and TEV

Expression

Bacterial

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b PKA Cat

Plasmid

#14921

Depositor

Susan Taylor

Article

Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only

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pCK054 pDEST exorh:mCerulean

Plasmid

#195985

Purpose

Transgenesis backbone

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-PGK-destination

Plasmid

#60436

Purpose

Destination vector with PGK - Hygromycin selection marker

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Expression

Mammalian

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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