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We narrowed to 4,839 results for: pcr

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Showing: 2141 - 2160 of 4839 results

  1. pTOPO-col10a1-KI-donor

    Plasmid
    #184874
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Publication
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Collagen10a1 5' homology arm
    Collagen10a1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    eGFP
    Use
    CRISPR and Cre/Lox
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    184874-plasmid-map-sequence-id-363155

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  4. pDisplay-NE2m-IRES-mCherry-CAAX

    Plasmid
    #208690
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells
    Depositor
    Yulong Li
    Publication
    Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    208690-plasmid-map-sequence-id-412239

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  5. pEM705 Gsi (Cys)

    Plasmid
    #109373
    Purpose
    GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)
    Depositor
    Robert Lucas
    Publication
    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gsi chimera
    Tags
    None
    Expression
    Mammalian
    Mutation
    Cys at position 391 (Pertussis Toxin Sensitive)
    Promoter
    CAG
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    109373-plasmid-map-sequence-id-206232

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  6. SSTR3-Tango

    Plasmid
    #66504
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SSTR3 (SSTR3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. CXCR6-Tango

    Plasmid
    #66264
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CXCR6 (CXCR6 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  8. BDKBR2-Tango

    Plasmid
    #66230
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BDKBR2 (BDKRB2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. C5A-Tango

    Plasmid
    #66232
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    C5A (C5AR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  10. ADRA2C-Tango

    Plasmid
    #66218
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ADRA2C (ADRA2C Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. ADCYAP1R1-Tango

    Plasmid
    #66208
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ADCYAP1R1 (ADCYAP1R1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  13. KISSPEPTIN-R-Tango

    Plasmid
    #66416
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KISSPEPTIN-R (KISS1R Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. mutpRL-TK 3xUTR

    Plasmid
    #40758
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. EDNRB-Tango

    Plasmid
    #66274
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EDNRB (EDNRB Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. GIPR-DuET

    Plasmid
    #213252
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GIPR (GIPR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213252-plasmid-map-sequence-id-430802

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  20. pcDNA3.1-GGamma4-GFP2

    Plasmid
    #166776
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma4-GFP2 (GNG4 Synthetic, Human)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166776-plasmid-map-sequence-id-330999

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Showing: 2141 - 2160 of 4839 results