We narrowed to 29,084 results for: ple

1. pLEX304-GFP-IQSEC1 v2-E620K GEF dead mutant

   Plasmid

   #162020

   Purpose

   Expression of GEF dead IQSEC1 mutant

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Insert

   IQSEC1 (IQSEC1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   EK

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro

   Plasmid

   #120560

   Purpose

   Expresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   HRAS (HRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   CMV

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti CMV-SLC7A11-sh926R-FLAG-IRES-Hygro

   Plasmid

   #118702

   Purpose

   lentiviral vector experessing SLC7A11 insensitive to hairpin TRCN0000288926

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   wt , SLC7A11 sh926R

   Promoter

   Available since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47870

   Purpose

   All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

   Depositor

   James Thomson, Erik Sontheimer

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Inserts

   NmCas9
   U6pr-tracrRNA
   OCT4 crRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLEX-uORF-HA-birA*-H-Ras(wildtype)-IRES-Puro

   Plasmid

   #120559

   Purpose

   Expresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   HRAS (HRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

   Plasmid

   #120564

   Purpose

   Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

   Plasmid

   #163644

   Purpose

   Makes a lentivirus that expresses mNeonGreen and Cas9.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   mNeonGreen-P2A-Cas9
   

   Use

   Lentiviral

   Tags

   mNeonGreen-P2A

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro

   Plasmid

   #177201

   Purpose

   encoding a dominant negative form of CEBP under inducible promoter

   Depositor

   Markus Covert

   Article

   Kudo et al bioRxiv (2022)

   Insert

   dnCEBP (A-C/EBP)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

   Plasmid

   #120563

   Purpose

   Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]

    Plasmid

    #159802

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc

    Plasmid

    #138368

    Purpose

    Lentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP

    Plasmid

    #69050

    Purpose

    lentiviral expression of IKZF3 with Q147H mutation and FLAG tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF3 (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    Q147H

    Promoter

    CAG

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hPlekhm1-Mid/pIRES puro Glue (Q513-E784)

    Plasmid

    #73455

    Purpose

    Express human middle part (Q513-E784) Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    CBP tag, HA tag, and strep tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE

    Plasmid

    #236241

    Purpose

    Lentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    CaV1.2 (CACNA1C Rabbit)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE

    Plasmid

    #236239

    Purpose

    Lentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    CaV2.1 (CACNA1A Rabbit)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE

    Plasmid

    #236240

    Purpose

    Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    CaV2.2 (CACNA1B Rabbit)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231367

    Purpose

    Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

    Plasmid

    #231368

    Purpose

    Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTwist-SARS-CoV-2 Spike-deltaC18 (P.1) Triple RBD

    Plasmid

    #212412

    Purpose

    Encodes SARS-CoV-2 spike K417T,E484K,N501Y for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike K417T,E484K,N501Y (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K417T,E484K,N501Y

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-Puro

    Plasmid

    #211622

    Purpose

    Rhno1 S141A

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    hRHNO1 (RHNO1 Human)
    

    Use

    Tags

    myc-flag

    Expression

    Mammalian

    Mutation

    S141A

    Promoter

    CMV

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge