We narrowed to 32,439 results for: Eng;

1. pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop

   Plasmid

   #169370

   Purpose

   Expression of gRNA in Culex quinquefasciatus or other mosquitoes

   Depositor

   Valentino Gantz

   Article

   Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

   Insert

   pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   CPIJ039596

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pILGFP84

   Plasmid

   #83542

   Purpose

   Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   SSA1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   SSA1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TU#1806_CRISPR_unc-73_exon21

   Plasmid

   #82360

   Purpose

   to create unc-73E null allele

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Tol2-UAS-Dendra2-miR-223 sponge

   Plasmid

   #97146

   Purpose

   For miR-223 knock down

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   miR-223 sponge
   

   Use

   Zebrafish

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. L1-1151Y>A

   Plasmid

   #13269

   Depositor

   Vance Lemmon

   Article

   Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

   Insert

   L1CAM (L1CAM Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

6. IRS1-B1

   Plasmid

   #25698

   Depositor

   Eric Fearon

   Article

   Bommer et al J Biol Chem. 2010 Jan 15. 285(3):1928-38

   Insert

   IRS1 (IRS1 Human)
   

   Use

   RNAi and Retroviral

   Available Since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pInducer21 Flag-Sesn1b

   Plasmid

   #61865

   Purpose

   Dox inducible lentivirial expression of Flag-Sesn1b (mouse)

   Depositor

   Ming Li

   Article

   Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

   Insert

   Sesn1 (Sesn1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Tol2-krt4-RFP-miR-223 sponge

   Plasmid

   #97150

   Purpose

   For miR-223 knock down

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   miR-223 sponge
   

   Use

   Zebrafish

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD

   Plasmid

   #73306

   Purpose

   Expresses mutant human PUM1-HD

   Depositor

   Traci Hall

   Article

   Wang et al Nat Methods. 2009 Nov;6(11):825-30. doi:

   Insert

   human PUM1 Pumilio homology domain (PUM1 Human)
   

   Tags

   intein

   Expression

   Bacterial

   Mutation

   Q867E-Q939E-C935S-Q1011E-C1007S

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pFL-TK-AID3'UTR-MUT

    Plasmid

    #19987

    Depositor

    Nina Papavasiliou

    Article

    Teng et al Immunity. 2008 May . 28(5):621-9.

    Insert

    AID 3' UTR (Aicda Mouse)
    

    Expression

    Mammalian

    Mutation

    Mutation

    Available Since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. IRS1-A4

    Plasmid

    #25697

    Depositor

    Eric Fearon

    Article

    Bommer et al J Biol Chem. 2010 Jan 15. 285(3):1928-38

    Insert

    IRS1 (IRS1 Human)
    

    Use

    RNAi and Retroviral

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pUASp-Flag-Myc-Qin-attB

    Plasmid

    #44208

    Depositor

    Phillip Zamore

    Article

    Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

    Insert

    Qin full length (qin Fly)
    

    Use

    Fly transformation

    Tags

    Flag and Myc

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pHRIG-S6K

    Plasmid

    #59446

    Purpose

    lentiviral expression of S6K and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al Stroke. 2014 Jul 10. pii: STROKEAHA.114.

    Insert

    S6K (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    S6K starts at aa24 and contains 7 extra amino aci…

    Promoter

    CMV

    Available Since

    Oct. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pSmBtA3-TrF

    Plasmid

    #245788

    Purpose

    Expression of mutated bovine arrestin-3 (L394Stop) tagged with SmBiT and Flag in mammalian cells

    Depositor

    Vsevolod Gurevich

    Article

    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

    Insert

    arrestin-3 (ARR3 Bovine)
    

    Tags

    Flag and SmBiT

    Expression

    Mammalian

    Mutation

    L394Stop

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSmBtA3-REF

    Plasmid

    #245786

    Purpose

    Expression of mutated bovine arrestin-3 (R170E) tagged with SmBiT and Flag in mammalian cells

    Depositor

    Vsevolod Gurevich

    Article

    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

    Insert

    arrestin-3 (ARR3 Bovine)
    

    Tags

    Flag and SmBiT

    Expression

    Mammalian

    Mutation

    R170E

    Promoter

    CMV

    Available Since

    Oct. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSmBtA3F

    Plasmid

    #245783

    Purpose

    Expression of bovine arrestin-3 tagged with SmBiT and Flag in mammalian cells

    Depositor

    Vsevolod Gurevich

    Article

    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

    Insert

    arrestin-3 (ARR3 Bovine)
    

    Tags

    Flag and SmBiT

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Oct. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQAT16

    Plasmid

    #223388

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNDGG005

    Plasmid

    #231309

    Purpose

    Vector part for BEVA; BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
    

    Expression

    Bacterial

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQAT15

    Plasmid

    #223387

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQAT17

    Plasmid

    #223389

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYPQAT23

    Plasmid

    #223395

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was driven by OsU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYPQAT26

    Plasmid

    #223398

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYPQAT28

    Plasmid

    #223400

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYPQAT29

    Plasmid

    #223401

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYPQAT30

    Plasmid

    #223402

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYPQAT14

    Plasmid

    #223386

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was by AtU3; BASTA for plant selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSCT221: pR6K-Donor(ZooTsy)_H1:12_E1:135_E2:20_H2:12

    Plasmid

    #200874

    Purpose

    ZooTsy donor harboring KanR cargo.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    ZooTsy
    

    Expression

    Bacterial

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSCT401: pHelper(AjTn6022)_QF-separated_TniQ-K361D

    Plasmid

    #200861

    Purpose

    Inducible expression of AjTn6022 proteins.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    AjTn6022
    

    Expression

    Bacterial

    Mutation

    TniQ-K361D

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSCT400: pHelper(AjTn6022)_QF-separated_TniQ-K348D

    Plasmid

    #200860

    Purpose

    Inducible expression of AjTn6022 proteins.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    AjTn6022
    

    Expression

    Bacterial

    Mutation

    TniQ-K348D

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMSIP103: pTarget(CyCAST)

    Plasmid

    #200849

    Purpose

    Target plasmid containing CyPSP1 and attachement site for CyCAST.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Inserts

    PAM for CyCAST + CyPSP1
    tRNA-Leu
    

    Expression

    Bacterial

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMSIP270: pHelper(CyCAST)_Shuffled-spacer_ΔTnsD

    Plasmid

    #200845

    Purpose

    Inducible expression of CyCAST proteins (except TnsD) with control crRNA for CyPSP1.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    CyCAST
    

    Expression

    Bacterial

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMSIP124: pDonor(CyCAST)_0.5kb-cargo

    Plasmid

    #200848

    Purpose

    CyCAST donor harboring non-functional cargo.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    CyCAST
    

    Expression

    Bacterial

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMSIP267: pHelper(CyCAST)_PSP1_ΔTniQ

    Plasmid

    #200842

    Purpose

    Inducible expression of CyCAST proteins (except TniQ) with crRNA targeting CyPSP1.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    CyCAST
    

    Expression

    Bacterial

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMSIP269: pHelper(CyCAST)_PSP1_ΔTnsD

    Plasmid

    #200844

    Purpose

    Inducible expression of CyCAST proteins (except TnsD) with crRNA targeting CyPSP1.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    CyCAST
    

    Expression

    Bacterial

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMSIP116: pHelper(CyCAST)_PSP1

    Plasmid

    #200840

    Purpose

    Inducible expression of CyCAST proteins with crRNA targeting CyPSP1.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    CyCAST
    

    Expression

    Bacterial

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMSIP122: pHelper(CyCAST)_Shuffled-spacer

    Plasmid

    #200841

    Purpose

    Inducible expression of CyCAST proteins with control crRNA for CyPSP1.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    CyCAST
    

    Expression

    Bacterial

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMZ103-rbs1-m3

    Plasmid

    #204465

    Purpose

    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127S mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Mutation

    LasR L125W-A127S mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMZ103-rbs1-m4

    Plasmid

    #204466

    Purpose

    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Mutation

    LasR L125W-A127T mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMZ103-rbs1-m5

    Plasmid

    #204467

    Purpose

    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125F-A127G-S129N mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Mutation

    LasR L125F-A127G-S129N mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMZ103-rbs1-m6

    Plasmid

    #204468

    Purpose

    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-S129N-L130V mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Mutation

    LasR L125W-S129N-L130V mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMZ103-rbs1-m7

    Plasmid

    #204469

    Purpose

    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T-L128M mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Mutation

    LasR L125W-A127T-L128M mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMZ103-rbs1-m8

    Plasmid

    #204470

    Purpose

    This is a biosensor with decreased specificity to 3OC12-HSL and improved specificity to 3OHC14-HSL, containing LasR L128F-S129W mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Mutation

    LasR L128F-S129W mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pNDGG007

    Plasmid

    #231311

    Purpose

    Vector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
    

    Expression

    Bacterial

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pNDGG001

    Plasmid

    #231307

    Purpose

    Vector part for BEVA; BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRExT2A-NT-BTy

    Plasmid

    #232206

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pRExT2A-CT-PTy

    Plasmid

    #232203

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-PAC-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pRExT2A-CT-BTy

    Plasmid

    #232209

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-BSD-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pRExT2A-NT-PTy

    Plasmid

    #232202

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    3xTy
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET-Cas9-FCPF

    Plasmid

    #220132

    Purpose

    Expresses Cas9-FCPF in E.coli

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    Cas9-FCPF (cas9 S. pyogenes)
    

    Tags

    His and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. dCas9-FCPF

    Plasmid

    #220131

    Purpose

    Expresses dCas9-FCPF in mammalian cells

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    dCas9-FCPF (cas9 S. pyogenes, Synthetic)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge