We narrowed to 2,878 results for: 623

1. psiCHECK-1-BRAF_Mut2-CM

   Plasmid

   #215923

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   one complete-matched (CM) sequence of siBRAF_ Mut2 (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   BRAF

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTFG002-ACE2(Mut)-HA

   Plasmid

   #191570

   Purpose

   ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(Mut)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA Tag.

   Depositor

   Joshua Leonard

   Article

   Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

   Insert

   Angiotensin Converting Enzyme II (ACE2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human expression. Also contai…

   Promoter

   CMV

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IP-muGFP-hATG2A mLIR

   Plasmid

   #215506

   Purpose

   Expresses GFP tagged human ATG2A with the mutation in LC3 interacting region.

   Depositor

   Noboru Mizushima

   Article

   Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

   Insert

   Autophagy related 2A (ATG2A Human)
   

   Use

   Retroviral

   Tags

   muGFP

   Expression

   Mammalian

   Mutation

   P656R (natural variant with no functional relevan…

   Promoter

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(ETS1(12))-PGKpuro2ABFP-W

   Plasmid

   #208550

   Purpose

   Lentiviral vector expressing gRNA targeting human ETS1

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   ETS1(12) (ETS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(ETS1(11))-PGKpuro2ABFP-W

   Plasmid

   #208549

   Purpose

   Lentiviral vector expressing gRNA targeting human ETS1

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   ETS1(11) (ETS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CL20_mEGFP_EP400_SFSWAP

   Plasmid

   #205810

   Purpose

   Express mEGFP-tagged fusion protein, EP400_SFSWAP from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   EP400_SFSWAP (EP400, SFSWAP Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Nmyc-Eps8L2_1-496-siR

   Plasmid

   #196515

   Purpose

   Expression of Myc-Eps8L2_1-496

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2_1-496 (EPS8L2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Silent mutations for siRNA resistance

   Promoter

   T7 promoter

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF1849

   Plasmid

   #143326

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ETS1 (ETS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1848

   Plasmid

   #142869

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ETS1 (ETS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis

    Plasmid

    #192835

    Purpose

    Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    3xFLAG-PreScission cleavage site and 6xhis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCVD293

    Plasmid

    #159765

    Purpose

    Expression vector for mutated Fc-fused ACE2(740)

    Depositor

    Jim Wells

    Article

    Glasgow et al Proc Natl Acad Sci U S A. 2020 Nov 10;11

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K31F/H34I/E35Q

    Promoter

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Bxb1Donor_G315T_ADRB2_LucReporter

    Plasmid

    #129793

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant G315T of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    G315T

    Promoter

    N/A

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Bxb1Donor_W99A_ADRB2_LucReporter

    Plasmid

    #129789

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    W99A

    Promoter

    N/A

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Bxb1Donor_N51L_ADRB2_LucReporter

    Plasmid

    #129790

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant N51L of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    N51L

    Promoter

    N/A

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET3d-SF-DCTN1-TS

    Plasmid

    #169375

    Purpose

    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

    Depositor

    Bernd Bukau

    Article

    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi

    Insert

    dynactin subunit 1 isoform 1 (DCTN1 Human)
    

    Use

    Tags

    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag

    Expression

    Bacterial

    Mutation

    Point mutations: A139T, I304T, Q322R, T516A, E577…

    Promoter

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX303-GFP-IQSEC1 v2N/v4C-WT

    Plasmid

    #162022

    Purpose

    Expression of IQSEC1 chimera

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    v2N/v4C

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX303-GFP-IQSEC1 v4N/v1C-WT

    Plasmid

    #162024

    Purpose

    Expression of IQSEC1 chimera

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    v4N/v1C

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX303-GFP-IQSEC1 v2N/v1C-WT

    Plasmid

    #162023

    Purpose

    Expression of IQSEC1 chimera

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    v2N/v1C

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-sACE2v2(732)

    Plasmid

    #154099

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (untagged)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-sACE2-T92Q-Fc(IgA1)

    Plasmid

    #145162

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) glycosylation mutant T92Q

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgA1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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