We narrowed to 2,854 results for: 623

1. pET-Cas9-6xHis

   Plasmid

   #62374

   Purpose

   Expression of Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9_6xHis
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW1512

   Plasmid

   #62366

   Purpose

   N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß

   Depositor

   Jonathan Weissman

   Article

   Jan et al Science. 2014 Nov 7;346(6210):1257521. d

   Insert

   3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE2

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJZC78

   Plasmid

   #62339

   Purpose

   sgRNA + 1x COM with COM-KRAB effector for mammalian cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 1x COM binding module
   COM-KRAB
   

   Use

   Lentiviral

   Tags

   KRAB

   Expression

   Mammalian

   Mutation

   Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

   Promoter

   CMV and U6

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACYC-LIC+

   Plasmid

   #62312

   Purpose

   E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis and thrombin protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET21a-LIC

   Plasmid

   #62303

   Purpose

   E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRE5

   Plasmid

   #62377

   Purpose

   Fluorescent reporter to quantify the ratio of two alternative splicing patterns.

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   dsRed and GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJZC34

   Plasmid

   #62331

   Purpose

   sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 2x MS2(wt+f6) binding module
   MCP-VP64
   

   Use

   Lentiviral

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

   Promoter

   CMV and U6

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRE5Cf

   Plasmid

   #62378

   Purpose

   Derived from FRE5. A two nucleotide insertion to shift the reading frame to dsRED.

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   dsRed and GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-gp78-FLAG

   Plasmid

   #62370

   Purpose

   Express C-terminally FLAG tagged gp78

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   gp78 (AMFR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   *

   Promoter

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

    Plasmid

    #62373

    Purpose

    Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
    

    Use

    Tags

    (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…

    Expression

    Bacterial

    Mutation

    Y67S amino acid substitution in (-30)GFP renders …

    Promoter

    T7

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBSKΔB- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo

    Plasmid

    #62346

    Purpose

    Expresses rtTA2sM2 and tTSkid in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    rtTA2sM2-IRES-tTSkid
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJZC74

    Plasmid

    #62342

    Purpose

    sgRNA + 1x COM with COM-KRAB effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1x COM binding module
    COM-KRAB
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJW1511

    Plasmid

    #62365

    Purpose

    pEF1a-puro-T2A-ATHmmuRPL10A

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    Puro-T2A-AVITEVHAmmuRPL10A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. MXS_chaining vector

    Plasmid

    #62394

    Purpose

    Empty Backbone

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Actin-mRFP-PAGFP

    Plasmid

    #62382

    Purpose

    Expresses beta actin with both a PAGFP and mRFP tag.

    Depositor

    Tim Mitchison, Guillaume Charras

    Article

    Kueh et al J Cell Biol. 2008 Jul 28;182(2):341-53.

    Insert

    beta actin (ACTB Human)
    

    Use

    Tags

    PAGFP and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. FERM domain of Ezrin

    Plasmid

    #62380

    Purpose

    Expresses the FERM domain of Ezrin (N terminal portion of the protein)

    Depositor

    Guillaume Charras

    Article

    Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

    Insert

    Ezrin aa1-309 (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains FERM domain of Ezrin (N terminal portion…

    Promoter

    CMV

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP

    Plasmid

    #62348

    Purpose

    An empty optimized gRNA expression vector

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    none
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDSAR-Cre-F2A-I-SceI

    Plasmid

    #62302

    Purpose

    Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Insert

    Cre recombinase, I-SceI nuclease
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    vasa

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJZC116

    Plasmid

    #62344

    Purpose

    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x MS2 (wt+f6) binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets CXCR4 , sequence: GCAGACGCGAGGAAGGAGGGCGC

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJW1517

    Plasmid

    #62387

    Purpose

    pFA6a Osm1(M33A)-yoEGFP::LEU2

    Depositor

    Jonathan Weissman

    Article

    Williams et al Science. 2014 Nov 7;346(6210):748-51. do

    Insert

    OSM1-EGFP fusion
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Methionine 33 mutated to Alanine

    Promoter

    Adh1p

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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