We narrowed to 13,573 results for: cas9 genes

1. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126706

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

   Insert

   ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

   Plasmid

   #160608

   Purpose

   TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   P35s:dCas9:SRDX:tNOS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. iF53 PB-Zim3-dCas9-mScarlet-Hygro

   Plasmid

   #204726

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-FLEX-SaCas9-U6-sgPenk

   Plasmid

   #159916

   Purpose

   Mutagenesis of Penk

   Depositor

   Larry Zweifel

   Insert

   Penk (Penk Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRM053 His-TEV-Cas9 triNLS 0C

   Plasmid

   #196244

   Purpose

   Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA, His6 (N terminal on backbone), NLS, Nucleopla…

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Promoter

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

   Plasmid

   #194063

   Purpose

   Mammalian expression of evoCjCas9 PEmax_deltaRnH

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-PEmax_dRnH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-dCas9-p300-P2A-PuroR

   Plasmid

   #83889

   Purpose

   Lentiviral Sp dCas9-p300-P2A-PuroR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
   pac from Streptomyces alboniger
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EFS

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD162 (Peft-3::Cas9 + Empty sgRNA)

   Plasmid

   #47549

   Purpose

   Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   Empty sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

   Plasmid

   #159905

   Purpose

   Mutagenesis of Slc32a1

   Depositor

   Larry Zweifel

   Insert

   Slc32a1 (Slc32a1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_TRE3G-p300-dCas9-P2A-mCherry

    Plasmid

    #138456

    Purpose

    2nd Generation Lentiviral vector. Expresses an N-terminal p300-dCas9 fusion protein and mCherry

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    p300 core
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2T-CMV-evoCjCas9-ABE8e-BlastR

    Plasmid

    #194060

    Purpose

    Mammalian expression of evoCjCas9 ABE8e

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-ABE8e
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV hUbC-Cas9-T2A-GFP

    Plasmid

    #53190

    Purpose

    3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-LTR-dCas9-VP64-BFP

    Plasmid

    #46912

    Purpose

    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    dCas9-VP64-BFP fusion
    Puromycin resistance
    

    Use

    CRISPR and Retroviral

    Tags

    3xNLS, BFP, and VP64 domain

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and PGK

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

    Plasmid

    #98971

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNA and spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLGR002 LGR Cas9 guide vector

    Plasmid

    #188320

    Purpose

    Universal backbone for CRISPR/Cas9 sgRNA expression

    Depositor

    Laralynne Przybyla

    Article

    Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    PuroR-T2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    pU6 5'CMV and EF1-alpha

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. UAS-FRT-GFP-FRT-u(M)Cas9

    Plasmid

    #127388

    Purpose

    Cas9 inducible by removal of a GFP flip-out cassette

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    FRT-GFP-FRT-uORF(M)Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1a-dCas9-KRAB-GFP backbone

    Plasmid

    #194281

    Purpose

    Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

    Plasmid

    #154139

    Purpose

    Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
    

    Use

    Tags

    3x FLAG and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-EF1alpha-VP64-dCas9-p300_Blast

    Plasmid

    #192654

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRE-KRAB-dCas9-IRES-BFP

    Plasmid

    #85449

    Purpose

    Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    KRAB-dCas9-IRES-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA Tag, and KRAB domain

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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