We narrowed to 2,383 results for: mia

1. pET28-SaCas9

   Plasmid

   #178901

   Purpose

   SaCas9 (Cas9 from Staphylococcus aureus)

   Depositor

   Hendrik Milting

   Article

   Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

   Insert

   SaCas9
   

   Use

   Tags

   His6-Tag, bipartite nuclear localization signal f…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ePPEmax*

   Plasmid

   #205244

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Promoter

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-PE2-SpG-P2A-GFP

   Plasmid

   #159980

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpG-P2A-GFP
   

   Use

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLTR.gp140/EGFP.Revdelta38/DsRed

   Plasmid

   #115775

   Purpose

   HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.

   Depositor

   Damian Purcell

   Article

   Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

   Insert

   Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
   

   Use

   Tags

   EGFP; dsReD

   Expression

   Mammalian

   Mutation

   gp140 uncleaved (1190-91aa, KR > TG; truncated…

   Promoter

   HIV-1 (LTR)

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS-H2B-cerulean

   Plasmid

   #53748

   Purpose

   Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.

   Depositor

   Sean Megason

   Article

   Megason et al Methods Mol Biol. 2009;546:317-32. doi:

   Insert

   histone H2B (H2BC21 Human)
   

   Use

   Tags

   Cerulean

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MoFGFR2c-ires neo

   Plasmid

   #86492

   Purpose

   mamalian cell expression of FGFR2c

   Depositor

   David Ornitz

   Article

   Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

   Insert

   FgfrR2 IIIc 15D6 (Fgfr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Moloney murine leukemia virus LTR

   Available since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS-H2B-mRFP1

   Plasmid

   #53745

   Purpose

   Contains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vector

   Depositor

   Sean Megason

   Article

   Megason et al Methods Mol Biol. 2009;546:317-32. doi:

   Insert

   histone H2B (H2BC21 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FLAG-ELL pBacPAK8

   Plasmid

   #15322

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

   Insert

   eleven nineteen lysine rich in leukemia (ELL Human)
   

   Use

   Baculovirus construction

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pET17b-yebST

   Plasmid

   #175804

   Purpose

   Complementation of yebST knockout in E. coli K-12

   Depositor

   Damian Ekiert, Ian Henderson, Gira Bhabha

   Article

   Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

   Insert

   yebST
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   silent mutations to introduce restriction sites

   Promoter

   T7

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPE3-pPuro

    Plasmid

    #215722

    Purpose

    Cloning vector for Uni-Vector prime editing system

    Depositor

    Kou-Juey Wu

    Article

    Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

    Insert

    PE2
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEYFP_C1_FANCM

    Plasmid

    #221384

    Purpose

    Expresses FANCM in mammalian cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Fanconi anemia group M protein (FANCM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2+MT-hToca-1-MGD

    Plasmid

    #33033

    Depositor

    Marc Kirschner

    Article

    Ho et al Cell. 2004 Jul 23;118(2):203-16.

    Insert

    Toca-1 (FNBP1L Human)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    MGD = amino acid substitutions M383I, G384S and D…

    Promoter

    simian CMV IE94

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pCS2+-H2B-pr-mEosFP

    Plasmid

    #141101

    Purpose

    H2B fused to pr-mEosFP for chromatin visualization and primed conversion

    Depositor

    Periklis Pantazis

    Article

    Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

    Insert

    Histone 2B
    

    Use

    Tags

    pr-mEosFP

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEN-R2-mCsfGFP-L3

    Plasmid

    #178602

    Purpose

    It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

    Depositor

    Frederica Theodoulou

    Article

    Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

    Insert

    Tandem fluorescent protein timers (tFTs)
    

    Use

    Entry vector for gateway cloning

    Tags

    mCherry superfolder GFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LB595 (Bcl-2 promoter CRE mut)

    Plasmid

    #15243

    Depositor

    Linda Boxer

    Article

    Heckman et al Oncogene. 2002 May 30. 21(24):3898-908.

    Insert

    B-cell lymphoma/leukemia-2 (BCL2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Bacterial

    Mutation

    Mutated CRE site at -1537 changed from GTGACGTTA …

    Promoter

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

16. FLAG-ELL2 pBacPAK8

    Plasmid

    #15327

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

    Insert

    Eleven Nineteen Lysine Rich in Leukemia 2 (ELL2 Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. GFP-Ago3m

    Plasmid

    #21531

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago3 (AGO3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    missing aa757-823, the C-termianl 66aa of PIWI do…

    Promoter

    Available since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

18. myc-SpELL pBacPAK8

    Plasmid

    #15318

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
    

    Use

    Baculovirus construction

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

19. NHA-Ago3m

    Plasmid

    #21532

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago3 (AGO3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    missing aa757-823, the C-termianl 66aa of PIWI do…

    Promoter

    Available since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pCS2+-Zeo-miR-430-G-G mm

    Plasmid

    #221728

    Purpose

    Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    BleoR-miR-430-G-G mm
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    G-G mismatch of miR-430 site

    Promoter

    simian CMV IE94

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge