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Showing: 1 - 20 of 2309 results

  1. pMIA10.7

    Plasmid
    #214451
    Purpose
    BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.
    Depositor
    Roger Foo
    Article
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Inserts
    Tet-On 3G
    PuroR
    TRE3G CopGFP
    Use
    Bxbi donor
    Tags
    PuroR, T2A, and Tet-On 3G
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214451-plasmid-map-sequence-id-431689

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  3. miABE

    Plasmid
    #205412
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205412-plasmid-map-sequence-id-416556

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  4. pMIA3

    Plasmid
    #109399
    Purpose
    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB
    Depositor
    Roger Foo
    Article
    Ang et al Cell Rep. 2018 Feb 20;22(8):2190-2205. d
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    mRuby2 and mtp53dn
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    109399-plasmid-map-sequence-id-211963

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  9. pMIA10.5-Clover

    Plasmid
    #214449
    Purpose
    BxbI landing-pad mClover donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.
    Depositor
    Roger Foo
    Article
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Insert
    mClover
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214449-plasmid-map-sequence-id-431685

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  13. pMIA10.53-Clover

    Plasmid
    #214450
    Purpose
    BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.
    Depositor
    Roger Foo
    Article
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Insert
    mClover
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214450-plasmid-map-sequence-id-431690

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Showing: 1 - 20 of 2309 results