-
Plasmid#115698PurposeER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiaeDepositorInsertSSKar2-sfpHluorin-HDEL
UseTagsER retention signal containing HDEL and ER signal…ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
DT044A
Plasmid#159405PurposeInducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)DepositorInsertpr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterT7/LacO promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRS-GFP
Plasmid#220197Purposemini-Tn7 GFP expression vectorDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpromoterlessAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG_mRuby2_smFP FLAG
Plasmid#59760Purposeencodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)DepositorInsertmRuby2_smFP_FLAG
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG_mRuby2_smFP OLLAS
Plasmid#59761Purposeencodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)DepositorInsertmRuby2_smFP_OLLAS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.GFPsm-myc.WPRE.SV40
Plasmid#98926PurposeAAV expression of GFP spaghetti monster myc constructDepositorInsertGFP-smFP-myc
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40
Plasmid#98927PurposeCre dependent AAV expression of GFP spaghetti monster myc constructDepositorHas ServiceAAV1InsertGFP-smFP-myc
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Ruby2sm-Flag.WPRE.SV40
Plasmid#98925PurposeAAV expression of Ruby2 spaghetti monster FLAG constructDepositorInsertRuby2-smFP-Flag
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRS-LacI-GFP
Plasmid#220198Purposemini-Tn7 GFP expression vector - LacI/pLac promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterLacI/pLacAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
SLC9A3R2_PDZ_2
Plasmid#103926PurposeProtein expression and purification of NHERF2 PDZ2 domainDepositorInsertSLC9A3R2 (NHERF2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRS-BG42-GFP
Plasmid#220202Purposemini-Tn7 GFP expression vector - pBG42 promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-BG35-GFP
Plasmid#220201Purposemini-Tn7 GFP expression vector - pBG35 promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-AraC-GFP
Plasmid#220199Purposemini-Tn7 GFP expression vector - AraC/pBAD promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterAraC/pBADAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-XylS-GFP
Plasmid#220200Purposemini-Tn7 GFP expression vector - XylS/pM promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterXylS/pMAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSGV-1D3-28Z All ITAMs intact
Plasmid#107226PurposeAnti-mouse CD19 CAR with CD3 ITAMs intactDepositorInsert1D3-28Z All ITAMs intact
UseRetroviralTagsNoneExpressionMutationNonePromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSGV1-1D3-28Z.1-3 mut
Plasmid#107227PurposeAnti-mouse CD19 CAR with CD3 ITAMs mutatedDepositorInsert1D3-28Z. 1-3 mut
UseRetroviralTagsNoneExpressionMutationNonePromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
Plasmid#98928PurposeCre dependent AAV expression of Ruby2 spaghetti monster FLAG constructDepositorHas ServiceAAV1InsertRuby2-smFP-Flag
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP
Plasmid#227009PurposeLentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domainDepositorInsertdCas9
UseCRISPR and LentiviralTags3xFLAG, SV40 NLSExpressionMutationPromoterUbCAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP
Plasmid#227010PurposeLentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domainDepositorInsertdCas9
UseCRISPR and LentiviralTags3xFLAG, SV40 NLSExpressionMutationPromoterUbCAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP
Plasmid#227011PurposeLentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domainDepositorInsertdCas9
UseCRISPR and LentiviralTags3xFLAG, SV40 NLSExpressionMutationPromoterUbCAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only