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Cas-Acr (insertion 5)

Plasmid

#128116

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (insertion 5)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (N39A)

Plasmid

#128117

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (N39A)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (N39A)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEBB FLAG XIAP D148A/W310A

Plasmid

#25685

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

FLAG

Expression

Mammalian

Mutation

D148A W310A

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pEBB HA XIAP delta BIR

Plasmid

#25686

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

HA

Expression

Mammalian

Mutation

delta BIR Contains only aa 331-497

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pEBB XIAP Delta RING

Plasmid

#40828

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Expression

Mammalian

Mutation

Delta RING: contains aa 1-448

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pEBB XIAP H467A

Plasmid

#25680

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Expression

Mammalian

Mutation

H467A

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pEBB XIAP D148A/W310A

Plasmid

#25678

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Expression

Mammalian

Mutation

D148A W310A

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
RV-Syn-Dio-GFP

Plasmid

#87673

Purpose

retroviral vector encoding cre dependent expression of GFP

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

eGFP

Use

Retroviral

Promoter

Synapsin

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (wtAcrIIA4)

Plasmid

#128115

Purpose

Expresses SpyCas9 fused to wild-type AcrIIA4

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (wtAcrIIA4)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA EMX1

Plasmid

#128121

Purpose

Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA EMX1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEBB FLAG XIAP D148A

Plasmid

#25683

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

FLAG

Expression

Mammalian

Mutation

D148A

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
RV-Cag-Dio-mTdt

Plasmid

#87665

Purpose

retroviral vector encoding cre dependent expression of membrane Tdtomato

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mem-Tdtomato

Use

Retroviral

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pEBB-HA-hILP del(2XBIR)

Plasmid

#25676

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

HA

Expression

Mammalian

Mutation

aa 262-497

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
RV-Cag-Dio-kikume

Plasmid

#87663

Purpose

retroviral vector encoding cre-dependent expression of kikume

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

kikume

Use

Retroviral

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pEBB XIAP W310A Delta RING

Plasmid

#25681

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Expression

Mammalian

Mutation

aa 1-449 only W310A

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pEBB FLAG XIAP W310A

Plasmid

#25684

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

FLAG

Expression

Mammalian

Mutation

W310A

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pNQ071

Plasmid

#242082

Purpose

Expresses E. coli RNAP (gene inserts: rpoA-rpoB-Nter-ybbR-rpoC-6xHis and rpoZ)

Depositor

Olivier Duss

Article

Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:

Insert

rpoA-rpoB-Nter-ybbR-rpoC-6xHis and rpoZ

Tags

His6

Expression

Bacterial

Mutation

ybbR-peptide tag inserted between E15 and E16 in …

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTBY1-T4gp61/41 fusion

Plasmid

#228210

Purpose

Expression in BL21(DE3) of T4 primase and helicase fusion with 24 amino acid linker

Depositor

Stephen Benkovic

Article

Manosas et al Nat Chem Biol. 2009 Dec;5(12):904-12. do

Insert

61/41

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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UPATrap-VC-ORF2

Plasmid

#110653

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Expression

Mammalian

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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UPATrap-VC-ORF1

Plasmid

#110652

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Expression

Mammalian

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge