We narrowed to 832 results for: pFAST

1. pFastBac_His-ZZ-TEV-Lis1

   Plasmid

   #132539

   Purpose

   Plasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tag

   Depositor

   Andrew Carter

   Article

   Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

   Insert

   Lis1 (PAFAH1B1 Human)
   

   Use

   Tags

   8x His, Linker-TEV-linker_TEV, and ZZ

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac1 Flag Baf170

   Plasmid

   #1955

   Depositor

   Robert Kingston

   Article

   Phelan et al Mol Cell 1999 Feb;3(2):247-53.

   Insert

   BAF170 (SMARCC2 Human)
   

   Use

   Tags

   flag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pFastBac-Dual_FluA/H7N9

   Plasmid

   #229860

   Purpose

   pFastBac Dual vector for co-expression of FluPolA/H7N9 polymerase heterotrimer (PA/PB1/PB2)

   Depositor

   Stephen Cusack

   Article

   Kouba et al Cell Rep. 2023 Jan 31;42(1):111901. doi:

   Inserts

   PA_H7N9-FluA
   PB1_H7N9-FluA
   PB2_H7N9-FluA
   

   Use

   Tags

   2x-Streptag and 8xHis-tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastbac1 Nterminal GST

   Plasmid

   #128058

   Purpose

   Baculovirus expression vector with N terminal GST

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFastBac1-LRRK2 WD40

   Plasmid

   #139211

   Purpose

   Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

   Depositor

   Hao Wu

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   N-His and MBP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastbac1-GST-hUSP7

   Plasmid

   #63573

   Purpose

   Expression of synthetic human USP7 tagged with GST

   Depositor

   Titia Sixma

   Article

   Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

   Insert

   USP7 (USP7 Synthetic, Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Synthetic based on human protein sequence (please…

   Promoter

   Polyhedrin

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG876 pDisplay-pFAST

   Plasmid

   #172868

   Purpose

   expresses pFAST at the surface of mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   pFAST-PDGFR
   

   Use

   Tags

   IgK leader sequence (N terminal on insert) and My…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac1 Flag BAF155 b

   Plasmid

   #1954

   Depositor

   Robert Kingston

   Article

   Phelan et al Mol Cell 1999 Feb;3(2):247-53.

   Insert

   BAF155 (SMARCC1 Human)
   

   Use

   Tags

   flag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pAG668 lifeAct-pFAST

   Plasmid

   #172866

   Purpose

   Expresses LifeAct-pFAST in mammalian cells (actin labeling)

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   LifeAct-pFAST
   

   Use

   Tags

   Myc tag (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac1_GI.1_N116C-G193C

    Plasmid

    #162580

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Asparagine 116 was mutated to Cysteine and Glycin…

    Promoter

    polyhedrin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBacI-KDAC8 H143A

    Plasmid

    #224344

    Purpose

    Expresses human KDAC8 (HDAC8) H143A in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC8 (HDAC8 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Histidine 143 mutated to alanine

    Promoter

    Polyhedrin

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pFastBacI-KDAC8 D101E

    Plasmid

    #224350

    Purpose

    Expresses human KDAC8 (HDAC8) D101E in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

    Insert

    KDAC8 (HDAC8 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Aspartate 101 mutated to glutamate

    Promoter

    Polyhedrin

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pFastBac1/MBP-His

    Plasmid

    #38008

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    MBP (Maltose binding protein)
    

    Use

    Baculovirus expression

    Tags

    6xHIS

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pFastBacDual with LFn + Needle

    Plasmid

    #84868

    Purpose

    Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    Needle
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac1_GI.1_Q62C-A140C

    Plasmid

    #162581

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Glutamine 62 changed to Cysteine and Alanine 140 …

    Promoter

    polyhedring

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG671 mito-pFAST

    Plasmid

    #172867

    Purpose

    Expresses mitochondrial targeting domain-pFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    mito-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG657 H2B-pFAST

    Plasmid

    #172862

    Purpose

    Expresses pFAST fused to zebrafish histone H2B in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    H2B-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastbac-Flag-BRCA1

    Plasmid

    #223228

    Purpose

    Insect expression construct for Flag-tagged BRCA1 protein production and purification

    Depositor

    Weixing Zhao

    Article

    Wang et al Mol Cell. 2023 Oct 19;83(20):3679-3691.e

    Insert

    Breast Cancer 1 (BRCA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBacDual with LFn + Fla

    Plasmid

    #84866

    Purpose

    Baculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    Fla
    

    Use

    Baculovirus

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac HTB DPP9L

    Plasmid

    #166788

    Purpose

    Insect cell expression vector for DPP9L (protein purification)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge