We narrowed to 594 results for: pFAST

1. pFastBac-His-Flag-Tipin-L195A

   Plasmid

   #23120

   Depositor

   Aziz Sancar

   Article

   Kemp et al J Biol Chem. 2010 Mar 15. ():.

   Insert

   Tipin (TIPIN Human)
   

   Use

   Tags

   Flag- and His

   Expression

   Insect

   Mutation

   Changed Leucine 195 to Alanine

   Promoter

   Available since

   March 3, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pFastBac-M13-8xHis-ZZ-LTLT-HOOK3

   Plasmid

   #222287

   Purpose

   Expression of HOOK3 in insect cells for purification of untagged adapter (8xHIS-ZZtag can be cleaved off with TEV protease).

   Depositor

   Anne Straube

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Insert

   HOOK3 (HOOK3 Human)
   

   Use

   Tags

   8 His and ZZ tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST

   Plasmid

   #223764

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac HTB His-TEV-rDPP9

   Plasmid

   #217326

   Purpose

   Purification of rat DPP9 from insect cells

   Depositor

   Yifan Wang

   Insert

   DPP9
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

   Plasmid

   #216378

   Purpose

   Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

   Depositor

   William Weis

   Article

   Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

   Insert

   Frizzled-4 (Fzd4 Mouse)
   

   Use

   Baculovirus

   Tags

   HA signal sequence-FLAG

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

   Plasmid

   #198037

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   E696K

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac-GST-TEV-mCherry-TBK1

   Plasmid

   #198033

   Purpose

   Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV and mCherry

   Expression

   Insect

   Mutation

   E696K

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac HTB MBP-SARM1 A600G

   Plasmid

   #195764

   Purpose

   Purification of SARM1 from insect cells

   Depositor

   Hao Wu

   Insert

   SARM1 (SARM1 Human)
   

   Use

   Tags

   HIS-MBP-TEV

   Expression

   Insect

   Mutation

   A600G

   Promoter

   polyhedrin

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBac-M13-6His-KIF1C_R169W-GFP

   Plasmid

   #185979

   Purpose

   Baculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

   Insert

   KIF1C (KIF1C Human)
   

   Use

   Tags

   6xHis and eGFP

   Expression

   Insect

   Mutation

   R169W - mutation causing HSP (SPG58)

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac-M13-6His-KIF1C_P176L-GFP

    Plasmid

    #185978

    Purpose

    Baculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    6xHis and eGFP

    Expression

    Insect

    Mutation

    P176L - mutation causing HSP (SPG58)

    Promoter

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBac1-OSF-TRIMCyp (owl monkey)

    Plasmid

    #79035

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5Cyp (owl monkey)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBac(His10-Lckg2a,Y394F,Y505F)

    Plasmid

    #177894

    Purpose

    Baculo expression of His10 tag fused to human LckG2A

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Lck (LCK Human)
    

    Use

    Tags

    10xHis-TEV

    Expression

    Insect

    Mutation

    G2A,Y394F,Y505F

    Promoter

    Polyhedrin

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac His6 TEV LIC cloning vector (4B)

    Plasmid

    #30115

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac Dual PNG-FLAG PLU-HIS

    Plasmid

    #113173

    Purpose

    Baculovirus expression of PNG and PLU

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    PNG, PLU
    

    Use

    Baculovirus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST

    Plasmid

    #223765

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BNIP3 soluble part (BNIP3 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFastBac Dual His6-TEV-CUL2, Rbx1

    Plasmid

    #231000

    Purpose

    Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Inserts

    Rbx1 (Rbx1 Mouse)
    CUL2 (Cul2 Mouse)
    

    Use

    Tags

    6x HIS

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac HTB His-TEV-rDPP9-FLAG

    Plasmid

    #217328

    Purpose

    Purification of rat DPP9 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    DPP9
    

    Use

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac HTB His-TEV-rDPP9(S729A)

    Plasmid

    #217327

    Purpose

    Purification of catalytically-dead rat DPP9 from insect cells

    Depositor

    Yifan Wang

    Insert

    DPP9
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    S729A

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac HTB His-TEV-BN-rNLRP1-FLAG

    Plasmid

    #217323

    Purpose

    Purification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    NLRP1
    

    Use

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac-Dual-GST-TEV-SINTBAD-mCherry

    Plasmid

    #208874

    Purpose

    For expression in insect cells of SINTBAD-mCherry

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge