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Plasmid#187723PurposeExpress His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cellsDepositorInsertNEDD4L residues 1-418+582-975 (NEDD4L Human)
UseTagsHis-TEVExpressionInsectMutationdeletion of residues 419-581PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_C942S_pCDNA3.1(+)
Plasmid#187729PurposeExpress the catalytically inactive NEDD4L FL in mammalian cellsDepositorInsertNEDD4L FL with C874 mutated to a serine (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationCys874 mutated to a serinePromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)
Plasmid#187708PurposeExpress his-tagged NEDD4L W2-W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 381-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-380PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT (492)
Plasmid#187709PurposeExpress his-tagged NEDD4L W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 492-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W4-HECT
Plasmid#187710PurposeExpress his-tagged NEDD4L W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 543-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-542PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_FL-CS
Plasmid#187713PurposeExpress the catalytically inactive GST-tagged NEDD4L FL in bacterial cellsDepositorInsertNEDD4L FL with C874 mutated to a serine (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationCys874 mutated to a serinePromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_W2-W3-W4-HECT (348)
Plasmid#187716PurposeExpress GST-tagged NEDD4L W2-W3-W4-HECT (348) in bacterial cellsDepositorInsertNEDD4L with residues XX (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-347PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_WW34
Plasmid#187718PurposeExpress GST-tagged NEDD4L WW34 tandem domains in bacterial cellsDepositorInsertNEDD4L residues 492-581 (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationonly residues 492-581PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_HECT
Plasmid#187719PurposeExpress GST-tagged NEDD4L HECT domain in bacterial cellsDepositorInsertNEDD4L residues 594-975 (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-593PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE311B
Plasmid#120418PurposeExpresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanRDepositorArticleInsertsMutL E32K
Lambda Beta
XylS
UseTagsExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterPmAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-control
Plasmid#213788PurposeCIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-Calm3-U6-control gRNA
UseLentiviralTagsGFPExpressionMammalianMutationPromoterSyn1, U6Available sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15-ZmPYK
Plasmid#220230PurposeInducible expression of Zymomonas mobilis pyruvate kinaseDepositorUseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKHH030_sgCtrl#1
Plasmid#174142PurposeLentiviral vector expressing a control sgRNA that targets a safe harbor siteDepositorInsertControl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_RB1#1
Plasmid#174150PurposeLentiviral vector expressing Cas9 and a sgRNA against the human RB1 geneDepositorInsertRB1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_sgCtrl#1
Plasmid#174148PurposeLentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor siteDepositorInsertControl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-Gas5
Plasmid#213172PurposeCIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-Calm3-U6-Gas5 gRNA
UseLentiviralTagsGFPExpressionMammalianMutationPromoterSyn1, U6Available sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_sgCtrl#2
Plasmid#174149PurposeLentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor siteDepositorInsertControl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 N C-V5-tag
Plasmid#179987PurposeMammalian expression vector for RaTG13 N, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 N
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKHH030_sgRB1#2
Plasmid#174145PurposeLentiviral vector expressing a sgRNA against the human RB1 geneDepositorInsertRB1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 M C-V5-tag
Plasmid#179986PurposeMammalian expression vector for RaTG13 M, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 M
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only