We narrowed to 36,325 results for: SHE

1. pFlpStop-HDR-UAS-2.1-tdTom

   Plasmid

   #89148

   Purpose

   FlpStop plasmid for CRISPR/HDR. Has MCS for homology arms

   Depositor

   Tom Clandinin

   Article

   Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Promoter

   5XUAS, 3XP3-dsRed

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SARS-CoV-2 Spike-d18

   Plasmid

   #164583

   Purpose

   A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

   Depositor

   Jason Sheltzer

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1256-1273

   Promoter

   EF1a

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SARS-CoV-2 Spike-d18

   Plasmid

   #164583

   Purpose

   A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

   Depositor

   Jason Sheltzer

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1256-1273

   Promoter

   EF1a

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSUPER.retro.puro-shNC

   Plasmid

   #113535

   Purpose

   Control retroviral vector.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   shNC (scrambled control shRNA)
   

   Use

   Retroviral

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSUPER.retro.puro-shNC

   Plasmid

   #113535

   Purpose

   Control retroviral vector.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   shNC (scrambled control shRNA)
   

   Use

   Retroviral

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRAF-MutL

   Plasmid

   #86607

   Purpose

   E. coli MutL expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutL
   

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRAF-MutL

   Plasmid

   #86607

   Purpose

   E. coli MutL expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutL
   

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-LambdaN-NbALFA

   Plasmid

   #207627

   Purpose

   Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

   Depositor

   Jay Shendure

   Article

   Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

   Insert

   LambdaN-NbALFA
   

   Use

   Synthetic Biology

   Promoter

   pCMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-LambdaN-NbALFA

   Plasmid

   #207627

   Purpose

   Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

   Depositor

   Jay Shendure

   Article

   Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

   Insert

   LambdaN-NbALFA
   

   Use

   Synthetic Biology

   Promoter

   pCMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-jRCaMP1a

    Plasmid

    #113936

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

    Depositor

    Robert Campbell, Thomas Hughes

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    jRCaMP1a
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-jRCaMP1a

    Plasmid

    #113936

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

    Depositor

    Robert Campbell, Thomas Hughes

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    jRCaMP1a
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUBC_HA_L7Ae-VP64

    Plasmid

    #68421

    Purpose

    Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    L7Ae
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Promoter

    human UbC

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUBC_HA_L7Ae-VP64

    Plasmid

    #68421

    Purpose

    Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    L7Ae
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Promoter

    human UbC

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRAF-MutH

    Plasmid

    #86609

    Purpose

    E. coli MutH expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutH
    

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRAF-MutH

    Plasmid

    #86609

    Purpose

    E. coli MutH expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutH
    

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRAF-MutS

    Plasmid

    #86605

    Purpose

    E. coli MutS expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutS (D835R,R840E)
    

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    D835R, R840E

    Promoter

    T7

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRAF-MutS

    Plasmid

    #86605

    Purpose

    E. coli MutS expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutS (D835R,R840E)
    

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    D835R, R840E

    Promoter

    T7

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201151

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    SadCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Mutation

    gRNA sequence: ATCAGTGATAGAGAACGTATG

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201151

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    SadCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Mutation

    gRNA sequence: ATCAGTGATAGAGAACGTATG

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_mCherry_2A_HA_2xMS2-VP64

    Plasmid

    #68419

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    MS2
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    MS2: V75E/A81G

    Promoter

    CMV

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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