...her that if she found something she loves to do, she would never work a day in her life. She said, “it ...open and collaborative.” She knew she wanted to leave academia, and once she joined an investment club...Cecchetelli knew she wanted to get her PhD. “Going back to school was a pretty easy choice for me,” she said. “...Operations Specialist, Lucy Hammer, about why she did not go. She told me, “I think that my experience is more...when she took her first job at Addgene as an Order Processing Assistant. After a few months, she left ...two years in graduate school, she realized that this was not her path. She told me, “it made more sense...undergraduate school and studying for the MCAT, she decided that she didn’t want to take it. “I realized if I...

...Susan panicked. She had always loved science, but didn’t know what she would do if she wasn’t at the bench... strength of Susan’s, but she didn’t know if she liked science writing. She decided to start a blog to...to grad school]!” He asked if she had considered other careers, which she hadn’t.  He then said, “How about...Institute (NEI), where she wrote about NEI research for a general audience. She then got a full time position... non-profit, like she did for the Northwest Association of Biomedical Research. She does, however, recommend...Ultimately, Susan feels she was lucky to have been in the right place at the right time when she started her scicomm... at the University of Maryland College Park, but she quickly realized it wasn’t the place for her. “I ...

...imaging, and more. Abil says she is currently working on a version that she hopes will change the intracellular...Assembly Kit out for themselves, she does have a couple of tips: First, she recommends using standard BsaI...The tool she and her colleagues led by Zhao described and demonstrated in a report published in The Journal...specific to any endogenous RNA without tagging,” she said. “It opens a new avenue of modifying and manipulating...changing bases to other RNA bases or unnatural bases,” she said. “The possibilities, I think, are endless.” ...hands, BsaI-HF has produced less reliable results. She also notes that for reasons that are not yet entirely...will do the same. “This isn’t the final version,” she said. “If we learn how to make the library even better...

...Validation Group and says that she decided to do a post-doc at Novartis because she wanted first-hand experience...experience in industry research. When she came to Novartis, she was happily surprised that “Novartis was actually...work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and she is ...came to Novartis for her post-doc in January 2012. She worked for three years as a post-doc in the Target...strong focus on publishing, but still made it so she could “get exposure to the drug discovery process...in cell line and primary tumor xenograft models. She hopes that the library can be used to identify appropriate...Bhang deposited the library with Addgene because she wanted to make it as easy as possible for researchers...

...candidate at the University of California, Irvine where she studies opioid use disorder. Her goal is to identify... of opioid withdrawal before they occur.  First, she used a genetic method to tag neurons activated by...withdrawal with tdTomato and assessed cFos expression. She will explore the connectivity patterns of these cells...chemogenetic agonist inhibits the neuron (via hM4D). She fused fluorescent protein tags to the receptors to... mechanical allodynia. With the dual regulation, she could then use pulses of blue light to activate the... the neurons at specific firing frequencies. She hopes to identify how in vivo firing frequency of PV ...While Qiu’s work on the PV neurons is in progress, she has validated the dual virus method to study CR neurons...

...and then fell in love with science,” she says. In 11th grade she heard a talk about Z DNA that inspired...technologies makes her a perfect fit for Addgene. She was Head of Quality at Dimension Therapeutics, which...the original employees at Dimension Therapeutics, she helped build and grow the gene therapy division of...inspired her to major in molecular biology in college. She then pursued a PhD in viral genetics. In regards ...share what they think will get the research funded,” she says. Baradaran met Addgene Executive Director Joanne...Association and it was through this connection that she learned more about Addgene. “The mission to share...

...turned on and off with light.  Xin hypothesized that she could make enzymes controllable by tethering them...oligomerize when exposed to ultraviolet light. To do so, she generated fusion proteins in which Dronpa domains...decrease the amount of light required for dimerization. She additionally showed that these enhanced “pdDronpa...advantage over other inducible enzyme technologies. She hopes that other researchers will be able to use ... J. Mansell’s lab at Iowa State University where she is developing fluorescent protein-based biosensors...displayed her findings at the Spring 2017 ACS meeting. She hopes these biosensors and her future work with HMOs...microbiomes in people all over the world. Toward this end, she recently presented her work at her home town in Bangladesh...

...for a quick refresher before you dive into the CRISPR eBook, check out the CRISPR Cheat Sheet. Download ...information. Download a PDF of CRISPR Cheat Sheet here!   CRISPR Cheat Sheet Clustered Regularly Interspaced Short...Originally published May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp...

... makes her the perfect Addgenie and she can’t wait to see how she can help the scientists in Europe with...of California, specialising in Molecular Biology. She has worked in a range of labs - cancer research, ... Imperial College London in Genetics. Since then she has worked in the the UK, California, Africa, and...viewing science as a global collaboration. This is why she is excited to be working at Addgene, because it brings...world to exchange research materials in an easy way. She has a passion for science, loving nothing more than...collaborators was extremely helpful, but with hindsight I wished I had gotten them from Addgene. I really struggled...

...ventral Developments in light sheet fluorescence microscopy Modern light sheet fluorescence microscopy was... of these advances in microscopy has been light sheet fluorescence microscopy (LSFM). The underlying method...).  The method was based on using a thin plane (sheet) of light generated by sunlight to observe single...Francis Spelman focused a laser beam into a thin sheet to illuminate a fluorescent sample and captured ...perpendicular to the plane of illumination (i.e. light sheet).  This is in contrast to confocal laser microscopy...for imaging live specimens, they combined light sheet microscopy with sequential multiple-view reconstruction...configurations’ various features see (4,7).   Light sheet fluorescence microscopy vs. confocal microscopy ...

...this system SHERLOCKv2 for SHERLOCK version 2. Here are some of the improvements: SHERLOCKv2 uses far less...Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg et al., 2017 and 2018). While.... Together SHERLOCK and DETECTR demonstrate the power of CRISPR-based diagnostics. SHERLOCK- Specific ... single base pair mismatch specificity.  SHERLOCKv2 SHERLOCK had a few limitations when it was first introduced...as an hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection system The ...flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK detection system, DETECTR...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection...

...coach who helps people in science and tech get jobs. She is also a very popular speaker and workshop leader... called “Secrets to Success in Graduate School”. She has also launched an online career coaching program...Beth Schachter Consulting is part of that group and she is especially excellent in coaching for communication...at Adeptify Me. While based in Madison, Wisconsin she also works remotely and has experience as a scientific...Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats, has an outstanding blog and... and, while her PhD is in History, I think she would be a good match as a coach for many scientists.  ...looks interesting and it is in English and Spanish. She is based in Spain. The bottom line – there are lots...

...Download Deposit Spreadsheet.” If you’re depositing 10 or more plasmids, the Deposit Spreadsheet is the quickest...and sending the deposit spreadsheet Figure 2: Example of the deposit spreadsheet showing the first eight...instructions will pop up. When you’re finished filling out the spreadsheet, save it in the format: labname_...Download a new spreadsheet each time you want to submit new plasmids! We update the spreadsheet every so often... more than 10 plasmids is to use our deposit spreadsheet?  Our plasmid deposit process can be broken down...this post, we’ll explain how to use our deposit spreadsheet to complete Step 1. Steps 2 and 3 are usually...send us your plasmid information in a deposit spreadsheet. The data entry process On the Addgene home...

...Have you ever heard of a Freshers Fair? If you’re from outside of Europe, the answer is probably no, ...opportunities available to them at university. At a Freshers Fair, all of the societies within a university... and recruit students to join their societies. Freshers Fairs tend to be used as welcome events for new... out useful items and information to students. Freshers fairs are also where many future scientists are...networks in their many years of study to come. Some Freshers Fairs can include fairground rides and other fun... some time to introduce you to the idea of the Freshers Fair and some of the great biological societies...Addgene has had the opportunity to work with at Freshers Fairs in the past year. Cambridge Biological Society...

...delivered by many of the speakers.  As Dr. Polka said as she started things off, “We are all capable of contributing...Postdoc Dilemma” which pretty much said it all.  She cited the oft quoted statistic that only 15% of current...postdocs will be academic principal investigators. She also pointed out promising new practices such as ...titled her presentation “How to Train Your PI”.   She focused her talk on the issues involved with the ...take advantage of all the possible science careers. She advocated for increasing the value and priority put...reproducibility being recognized in published work even in the “best” journals.  He pushed for drastic change in the...

...alexandrazgreenbaum.wordpress.com or on twitter @green__algae though she admits she doesn't tweet enough.  Nashville, TN, USA 36.1627...Canada). She works in a lab that uses yeast cells to study how DNA damage promotes cancer. She is also ...University in Montreal, Canada.  Outside the lab, she dabbles in science policy, public outreach, and restorative... University of Michigan Department of Astronomy. She studies how planets form around young stars and the...a post-doctoral fellow at Vanderbilt University. She studies beta-cell function and failure in Type 2 ...Colorado Anschutz Medical Campus in Aurora, CO where she studies the development and regeneration of mammalian...truth. I also, it turns out, marched with Truth. She was a woman in a lab coat.  The march opened with...

...School where she brought leadership teams together to advance the mission of the school. She was also a ...Harvard Medical School, Joanna was my colleague. She mentioned Addgene was looking for someone with HR...recent foster dog was with us for two months before she found her forever home. I feel blessed that I’m not...

... Cell Stem Cell.  She was the founding Editor-in-Chief of Cell Stem Cell, a role she held for 10 years... the Vice President of Editorial at Cell Press.  She trained originally in cell biology, completing her...Scripps Research Institute in La Jolla, California. She joined Elsevier after her postdoctoral studies, and...Metabolism, Cell Reports and Joule. In September 2017 she moved away from direct editorial work to focus on...communication falls on the shoulders of journals and publishers. As journal editors, we often find ourselves ...contributions, etc.) before their paper can be published. To a scientist, all of this checking and formatting...clear information about reagents, and why other publishers and organizations have various different versions...

...Currently, she is a postdoc at KAUST, Saudi Arabia, in the group of Prof. Mark Tester, where she focuses ...using R/Shiny. She is passionate about open science, BIG data analysis and sharing whatever she knows with...with whoever cares to listen. In March 2020 she will start her own research group at Boyce Tompson Institute...touchstone of the scientific method, ensuring that published work meets the accepted standards of reporting...

...but after receiving her degree she was faced with a similar conundrum – she had the skills, training, and...Technology in 2004, she accepted a commission in the United States Air Force. She left active duty to ...Keira had it a little better. As a Master’s student, she had less pressure to publish and more freedom to ...his two cats. Keira Havens grew up in Hawaii where she was fascinated by flowers, bugs, and the ocean. After...intellectual property, it worked away in isolation. It published a paper a year (or less) and limited the exchange...