...current projects in the works, and all of the things she loves about working at Addgene! Maria Soriano Maria...

... but if available, use an automated ELISA plate washer. Equipment Spectrophotometer compatible with 96...controller Microcentrifuge Microplate shaker ELISA plate washer (optional) Scale Microsoft Excel or similar software... but if available, use an automated ELISA plate washer. Seal the plate. Place the plate on a microplate... . Repeat steps 8–10 twice for a total of three washes. After the final wash, aspirate the wash buffer... but if available, use an automated ELISA plate washer. Seal the plate Place the plate on a microplate.... Repeat steps 18–20 twice for a total of three washes. After the final wash, aspirate the wash buffer...

... Biohazard waste container Fire blanket Fire extinguisher Guidelines BSL-1 Guidelines Before You Work ...eyewash station, safety shower, fire blanket, and extinguisher are also required to be present in the room....station if unwanted or biohazardous materials are splashed onto your face and/or get into your eyes. Wash...chemicals, first review their material safety data sheets (MSDS). While You Work Dispose of sharps in a designated...shaking or mixing, which may produce aerosols or splashes. These procedures should be done underneath a ...

...antibody, 0.9–1.1 mg/mL SimplyBlue SafeStain, Thermo Fisher, LC6060 4-12% NuPage Novex bis-tris mini gel, 1mm... LC Area column for the IgG standards in Google Sheets. Open exported CSV file. Select import. Choose ... Chart . This will add a blank chart to your spreadsheet. In the Chart Editor , select the Chart Type ...Add a linear trendline for the standards. Google Sheets can calculate this for you as follows: In the Chart...equation and the R 2 of the trendline in Google Sheets as follows: In the Customize tab, select the Label...

... buffer Microcentrifuge tubes BCA Assay, Thermo Fisher 23227 β-mercaptoethanol 4X protein loading buffer... ladder Ethanol iBlot 2 PVDF Mini Stack, Thermo Fisher IB24002 20X TBS Tween-20 Nonfat milk powder 96-...deionized water 3x . Shake the gel gently between washes to remove residual water. Load the gel into the...

...-80°C. Subsequent freeze and thaw cycles reduce shelf life. To recover bacteria from your glycerol stock...it from thawing completely and will improve the shelf life. It is very important that you shake the glycerol...

...infection and selection G. Safety H. References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence...Do not use a 0.2 μm filter, as this is likely to shear the envelope of your virus. o. Virus may be stored...Biosafety Guide . Back to Top H. References H.1. Published Articles Functional siRNAs and miRNAs exhibit ...

...exposure. Read the reagent’s material safety data sheet (MSDS) to determine if this is best. Check that ...biohazardous. Check your lab’s material safety data sheets (MSDS) to understand how to properly dispose of...

...pipette, and the tip ejector ring at the bottom which pushes the pipette tip off of the pipette. This side view...rate into the pipette tip. Once the liquid has finished drawing into the pipette tip, lift the pipette...

... system, 500 mL, VWR 430770 Trypan Blue, Thermo Fisher T10282 Valproic acid sodium salt, Sigma Aldrich... Grade, VWR 75800-188 10% Pluronic F-18, Thermo Fisher 24040032 Glutagro, Corning 25-015-CI BalanCD HEK293...

...luer-lock), VWR 431229 IgG Elution Buffer, Thermo Fisher 21028 Protein G GraviTrap columns, Cytiva 28-9852...Spin Desalting Columns, 7 kDa MWCO, 10 mL, Thermo Fisher 89893 Amicon Ultra-15 30 kDa MWCO column, Millipore...

...of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher 12605-010) 6-well tissue culture treated dish, ...trypsinize the cells and expand them to larger culture dishes, being sure not to cross contaminate the independent...

...opens in a new window) TRIzol® User Guide from ThermoFisher Scientific . Figure 1: A diagram of the different... Caution Make sure to read the SDS (Safety Data Sheet) for safety warnings and hazards for these reagents...

...) Opti-MEM, Thermo Fisher 31985-070 0.05% Trypsin/EDTA (e.g. TrypLE, Thermo Fisher, 12605010) 1X PBS pH...

...Reagents GeneJet Genomic DNA Purification Kit, Thermo Fisher, K0721 6-well tissue culture treated dish, VWR,...of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher, 12605010) Ethanol, VWR, EX0276-1 Benzonase 250...

...of T4 Pol in the following step. This may be accomplished by gel purification . Step 5: Create Insert ...

...centrifuge and investigate the issue. When you are finished spinning your samples, carefully remove them from...

...medium such as Opti-MEM or Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear...

...compatibly digested vector backbone. This is accomplished by covalently connecting the sugar backbone ...

...medium such as Opti-MEM or Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear...