...platform, termed SHERLOCK, can identify target sequences using a fluorescent reporter. In SHERLOCK, a quenched... the presence of that specific target. SHERLOCK (and SHERLOCKv2) allows for greater amplification and ...bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the Feng Zhang lab , is a Cas13a-based...flow strips. Other Cas enzymes can be added to SHERLOCKv2 to detect multiple targets. For example, Cas12...Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & ...system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman, E., Younger, S. T., & Rinn...

...The insert is typically the genetic element one wishes to study using a particular plasmid. Promoter Region...expression of an endogenous gene. This is frequently accomplished through expression of an shRNA targeting the...edit genomes. Genome editing is most commonly accomplished using CRISPR technology. CRISPR is composed ...concentrations. We suggest checking your plasmid's datasheet or the plasmid map to confirm which antibiotic...with a different fluorophore so they can be distinguished from one another. Similar to DNA replication...

... Prigge M, Schneider F, Davidson TJ, O'Shea DJ, Sohal VS, Goshen I, Finkelstein J, Paz JT, et al. 2011...Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I, Thompson KR, Deisseroth K. 2010 Molecular and... Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M, Fenno LE, Gradinaru...

...the number of signaling molecules that can be distinguished in your application. The indirect detection ...Cross-link the samples with formaldehyde or UV light. Shear the samples using sonication to break DNA up into...protein. Micrococcal nuclease digestion is used to shear the DNA in these assays. This method is suitable...specific to your antibody and assay may already be published. If it is not, you’ll need to validate the antibody...

...onto the insert of interest using PCR. This is accomplished by PCR amplifying the insert sequence using ...round of reverse transcription. This deletion abolishes transcription of the full-length virus after it...

...System? The laboratory of Bert Vogelstein has published their method for generation of recombinant adenoviruses...

...relevant to the phenotype being studied. Examples of published selection mechanisms for lentiviral CRISPR libraries...

...for development into chemogenetic tools. Need a refresher on G-protein coupled receptors? Read the GPCRs...