We narrowed to 14,156 results for: gan
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Plasmid#245834Purposeexpresses TAPBPR-S104F-K211L-R270Q in S2 cellsDepositorInsertTAPBPR (S104F K211L R270Q) (TAPBPL Human)
TagsAviTag biotinylation tag and His6 affinity tagExpressionInsectMutationS104F K211L R270Q (HiFi mutants) & C94S (remo…PromoterMT_puroAvailable SinceOct. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2
Plasmid#218557PurposeEncodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistanceDepositorInsertMCP-HSF1-VIRF2
UseLentiviralTagsSV40NLSExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…Available SinceAug. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
BPK1520-sgRNA GLB1
Plasmid#184378PurposeExpresses a sgRNA to edit GLB1 gene NM_000404.2_c.907A>GDepositorAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA)
Plasmid#240228PurposeParental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.DepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)
Plasmid#240229PurposeParental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.DepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUAS-hhN-HA
Plasmid#240236PurposeExpression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.DepositorInserthedgehog N-terminal fragment (hh Fly)
TagsHA-spGFP11ExpressionInsectMutationaa 1-257PromoterUASAvailable SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His
Plasmid#222288PurposeExpression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.DepositorInsertKIF1C (KIF1C Human)
Tags6xHis, GFP, and GSTExpressionBacterialMutationstalk region only amino acids 642 to 922PromoterT7Available SinceJune 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b-GST-6P2-LTLT-KIF1C(642–922)-6His
Plasmid#222289PurposeExpression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal 6xHis tag in E.coli for purification.DepositorInsertKIF1C (KIF1C Human)
Tags6His and GSTExpressionBacterialMutationstalk region only amino acids 642 to 922PromoterT7Available SinceJune 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
DHFR-C-HiBiT
Plasmid#207119PurposeBacterially expressed DHFR-HiBiT for Ni-NTA purificationDepositorInsertHuman-dihydrofolate reductase-HiBiT-10xHis (DHFR Human)
Tags10xHis-tag and HiBiT tagExpressionBacterialMutationCodon optmization for expression in BL21DE3PromoterT7Available SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori
Plasmid#234737PurposeAll-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.DepositorAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only