We narrowed to 13,683 results for: pcDNA3

1. pCDNA3 IL-2 signal mFc

   Plasmid

   #128042

   Purpose

   Mammalian expression vector with C terminal IL-2 signal peptide and mFc

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Tags

   IL-2 signal peptide and mFc

   Expression

   Mammalian

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3 Flag Jnk1b1

   Plasmid

   #13753

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk1b1 (MAPK8 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1-CibN-dCas9

   Plasmid

   #60551

   Purpose

   Expresses CibN-dCas9 in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   CibN-dCas9
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-MS2-HA-HsNot4_AA

   Plasmid

   #148255

   Purpose

   Mammalian Expression of HsNot4

   Depositor

   Elisa Izaurralde

   Article

   Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

   Insert

   HsNot4 (CNOT4 Human)
   

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Available Since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-RsSHC014-RBD-8h

   Plasmid

   #161826

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-pri-let-7g

   Plasmid

   #51381

   Depositor

   Narry Kim

   Article

   Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

   Insert

   pri-let-7g (MIRLET7G Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/V5-His-TN-DROSHA

   Plasmid

   #106252

   Purpose

   Express TN-DROSHA in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Chang et al Genome Res. 2015 Sep;25(9):1401-9. doi:

   Insert

   Drosha Ribonuclease III (DROSHA Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   E1045Q and E1222Q mutations

   Promoter

   CMV

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-SARS-1-RBD-8h

   Plasmid

   #161821

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T320-D518)

   Promoter

   CMV

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-neo-Strep_Flag_Cterm

   Plasmid

   #102645

   Purpose

   Epitope tag c-terminus with SBP-Flag moiety

   Depositor

   Michael E. Wright

   Article

   Hsiao et al BMC Cancer. 2015 Mar 31;15:204. doi: 10.

   Type

   Empty backbone

   Tags

   SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

   Expression

   Mammalian

   Available Since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-mRuby2-MACROD1_d77

    Plasmid

    #172588

    Purpose

    For transient expression of C-terminal mRuby2-tagged MACROD1del1-77

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-76

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid

    #92303

    Purpose

    Expression of humanized TsCpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huTsCpf1
    

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+) Drp1 V2 S579D

    Plasmid

    #46343

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    Drp1 V2 (Dnm1l Mouse)
    

    Expression

    Mammalian

    Mutation

    S579D

    Promoter

    CMV

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-Firefly-ccdB

    Plasmid

    #87069

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal Firefly luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Carl-YeNL-KDEL_pcDNA3

    Plasmid

    #89516

    Purpose

    Yellow color variant of bright luminescent protein, targeted to the endoplasmic reticulum

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    YeNL
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.2-V5-DEST-hSFRP1-VF

    Plasmid

    #189542

    Purpose

    Protein expression of SFRP1 in mammalian cells.

    Depositor

    Haian Fu

    Insert

    secreted frizzled related protein 1 (SFRP1 Human)
    

    Tags

    VF

    Expression

    Mammalian

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP

    Plasmid

    #67299

    Purpose

    Blue light dependent photoswitching system

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    Tiam1-pMagFast2(3x)-iRFP (Tiam1 Synthetic, Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Carl-CeNL-KDEL_pcDNA3

    Plasmid

    #89509

    Purpose

    Cyan color variant of bright luminescent protein, targeted to the endoplasmic reticulum

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-FluoSTEP-JNKAR

    Plasmid

    #181857

    Purpose

    Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-JNKAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-FluoSTEP-AktAR

    Plasmid

    #181856

    Purpose

    Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-AktAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 EYFP H148Q

    Plasmid

    #25871

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q
    

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with Histidine 148 to …

    Available Since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only