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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 27,993 results for: ung

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Showing: 2201 - 2220 of 27993 results

  1. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72248-plasmid-map-sequence-id-131868

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  5. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid
    #101178
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/H698A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101178-plasmid-map-sequence-id-219907

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  13. MSP1101

    Plasmid
    #65773
    Purpose
    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, and T1337R mutations in C…
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65773-plasmid-map-sequence-id-112657

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  16. BPK2797

    Plasmid
    #72251
    Purpose
    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, and T1337R
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72251-plasmid-map-sequence-id-131872

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Showing: 2201 - 2220 of 27993 results